Trifluralin herbicide-induced resistance of melon to fusarium wilt involves expression of stress- and defence-related genes

• Published in: Molecular plant pathology Vol. 8; no. 1; pp. 9 - 22
• Main Authors: LOTAN-POMPAN, MAYA, COHEN, RON, YARDEN, ODED, PORTNOY, VITALY, BURGER, YOSEF, KATZIR, NURIT
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 2007; Blackwell Publishing Ltd; Blackwell
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fusarium oxysporum; Genetics and breeding of economic plants; Melonis; Nitro compound; Plant pathogen; Mycosis; Cucurbitaceae; Pesticides; Induced resistance; Herbicide; Fungus resistance; Stress; Fungi; Infection; Gene; Fruit crop; Dicotyledones; Angiospermae; Defense mechanism; Spermatophyta; Trifluralin; Fungi Imperfecti; Cucumis melo; Thallophyta
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/j.1364-3703.2006.00365.x

To identify genes involved in trifluralin herbicide-induced resistance of melon to Fusarium oxysporum f. sp. melonis, suppression subtractive hybridization (SSH) and cDNA-amplified fragment-length polymorphism (cDNA-AFLP) were used. A total of 123 clones--60 of which have never been isolated from melon--were isolated, sequenced and annotated. A significant proportion (35%) of the total 123 clones exhibited similarity to genes that have been formerly described as stress- or defence-related. Thirty-two selected clones were subjected to a detailed expression analysis, one-third of which were found to be up-regulated in response to trifluralin treatment and/or fusarium inoculation. The putative roles of seven of these clones in stress are discussed. Furthermore, the expression of four stress-related and up-regulated genes was enhanced when the plants were subjected to salinity stress, suggesting that trifluralin induces a general stress response which protects the plant against fusarium wilt.