Purification and Identification of Peptides from Oyster ( Crassostrea hongkongensis ) Protein Enzymatic Hydrolysates and Their Anti-Skin Photoaging Effects on UVB-Irradiated HaCaT Cells

• Published in: Marine drugs Vol. 20; no. 12; p. 749
• Main Authors: Peng, Zhilan, Gao, Jialong, Su, Weimin, Cao, Wenhong, Zhu, Guoping, Qin, Xiaoming, Zhang, Chaohua, Qi, Yi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.11.2022; MDPI
• Subjects: Analysis; Animals; Bax protein; Bcl-2 protein; Cells; characterization; Chromatography; Collagen; Collagen (type I); Cosmetics; Crassostrea; Crassostrea - chemistry; Crassostrea hongkongensis; HaCaT Cells; High performance liquid chromatography; HPLC; Humans; Hydrogen bonding; Hydrogen bonds; Hydrolysates; Identification and classification; Interstitial collagenase; Liquid chromatography; MAP kinase; Marine molluscs; Matrix metalloproteinase; Matrix Metalloproteinase 1 - metabolism; Molecular docking; Molecular Docking Simulation; Molecular weight; NF-kappa B - metabolism; NF-κB protein; Oligopeptides - chemistry; Oligopeptides - isolation & purification; Oligopeptides - pharmacology; Oxidative stress; oyster; Oysters; peptide; Peptides; Phosphorylation; photoaging; Properties; Protein purification; Proteins; purification; Signal transduction; Skin; Skin Aging - drug effects; Tandem Mass Spectrometry; Ultrafiltration; Ultraviolet Rays - adverse effects; Water purification; China; oyster; peptide; characterization; purification; photoaging
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20120749

This study aimed to purify and identify antiphotoaging peptides from oyster ( ) protein enzymatic hydrolysates (OPEH) and to investigate the possible mechanism underlying its antiphotoaging effect. Multiple methods (Ultrafiltration, G25 Chromatography, RP-HPLC, and LC/MS/MS) had been used for this purpose, and eventually, two peptides, including WNLNP and RKNEVLGK, were identified. Particularly, WNLNP exerted remarkable antiphotoaging effect on the UVB-irradiated HaCaT photoaged cell model in a dose-dependent manner. WNLNP exerted its protective effect mainly through inhibiting ROS production, decreasing MMP-1 expression, but increasing extracellular pro-collagen I content. Furthermore, WNLNP downregulated p38, JNK, ERK, and p65 phosphorylation in the MAPK/NF-κB signaling pathway and attenuated bax over-expressions but reversed bcl-2 reduction in UVB- irradiated HaCaT cells. The molecular docking analysis showed that WNLNP forms five and seven hydrogen bonds with NF-κB (p65) and MMP-1, respectively. This study suggested that a pentapeptide WNLNP isolated from OPEH had great potential to prevent and regulate skin photoaging.