High-yields heterologous production of the novel Aspergillus fumigatus elastase inhibitor AFUEI in Aspergillus oryzae

AFUEI, an elastase inhibitor produced by Aspergillus fumigatus strongly inhibits the elastolytic activity of A. fumigatus etc. To purify AFUEI, we constructed a strain that overproduces AFUEI by introducing the gene encoding AFUEI (Genbank accession no. AB546725) under control of the amyB promoter i...

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Published inJournal of bioscience and bioengineering Vol. 112; no. 2; pp. 114 - 117
Main Authors Yamashita, Nobuo, Komori, Yumiko, Okumura, Yoshiyuki, Uchiya, Kei-ichi, Matsui, Takeshi, Nishimura, Akira, Ogawa, Kenji, Nikai, Toshiaki
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.08.2011
Elsevier
Subjects
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
Aspergillus fumigatus
Aspergillus fumigatus - enzymology
Aspergillus oryzae
Aspergillus oryzae - genetics
Biological and medical sciences
Biotechnology
Cells, Cultured
Clinical trials
Elastase
Elastase inhibitior
Fundamental and applied biological sciences. Psychology
Fungal Proteins - biosynthesis
Fungal Proteins - genetics
Fungal Proteins - pharmacology
Heterologous production
Humans
Pancreatic Elastase - antagonists & inhibitors
Promoters
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - pharmacology
Serine Proteinase Inhibitors - biosynthesis
Serine Proteinase Inhibitors - genetics
Serine Proteinase Inhibitors - pharmacology
Ultrafiltration
Heterologous production
Aspergillus oryzae
Aspergillus fumigatus
Elastase inhibitior
Fungi
Production
Inhibitor
Yield
Fungi Imperfecti
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Summary:AFUEI, an elastase inhibitor produced by Aspergillus fumigatus strongly inhibits the elastolytic activity of A. fumigatus etc. To purify AFUEI, we constructed a strain that overproduces AFUEI by introducing the gene encoding AFUEI (Genbank accession no. AB546725) under control of the amyB promoter into the heterologous host Aspergillus oryzae. A. oryzae TF-4 displayed strong elastase inhibitory activity and produced considerably more AFUEI than that of A. fumigatus. Furthermore, AFUEI could be purified using culture broth and single ultrafiltration (UF) treatment, allowing for the effective production of AFUEI for use in clinical trials.
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ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2011.03.020