Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream ( Sparus aurata), rainbow trout ( Oncorhynchus mykiss) and common carp ( Cyprinus carpio)

• Published in: Biochimica et biophysica acta Vol. 1474; no. 1; pp. 61 - 69
• Main Authors: Panserat, S., Blin, C., Médale, F., Plagnes-Juan, E., Brèque, J., Krishnamoorthy, J., Kaushik, S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 06.03.2000; Elsevier
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Base Sequence; Carps; Carps - genetics; chemistry; Cloning, Molecular; complementary DNA; Dietary carbohydrate; DNA, Complementary; DNA, Complementary - chemistry; Fish nutrition; genetics; glucokinase; Glucokinase - genetics; Glucose phosphorylation; Life Sciences; Molecular Sequence Data; nucleotide sequences; Oncorhynchus mykiss; Oncorhynchus mykiss - genetics; Perciformes; Perciformes - genetics; Phylogeny; Sequence Alignment; Dietary carbohydrate; Fish nutrition; Glucose phosphorylation; DAURADE ROYALE; ALIMENTATION DES POISSONS; CARPE COMMUNE
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(99)00213-5

The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream ( Sparus aurata) and rainbow trout ( Oncorhynchus mykiss) than in common carp ( Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from these cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly homologous to mammalian GK exists in these fish species with similar tissue specific expression (mainly liver).