Haploid plantlet production through somatic embryogenesis in anther-derived callus of Bupleurum falcatum

• Published in: Plant production science Vol. 7; no. 2; pp. 204 - 211
• Main Authors: Shon, T.K. (Kyungpook National Univ., Taegu (Korea R.)), Kim, S.K, Acquah, D, Lee, S.C
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 2004; Crop Science Society of Japan; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Agronomy. Soil science and plant productions; ANTHER CULTURE; Biological and medical sciences; BUPLEURUM; Bupleurum falcatum; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Genetics and breeding of economic plants; Haploid; HAPLOIDY; Haploidy, in vitro culture applications, somatic hybrids; Plant breeding: fundamental aspects and methodology; SOMATIC EMBRYOGENESIS; Somatic embryogensis; Androgenesis; Plant production; Anther; Embryonic development; Haploidy; Callus; Haploid; Somatic embryogensis; Plantlet; Anther cultures Bupleurum falcatum; Somatic embryo
• ISSN: 1343-943X; 1349-1008
• DOI: 10.1626/pps.7.204

This study was carried out to verify the production of haploid plantlets through somatic embryogenesis of Bupleurum falcatum in anther culture (2n=16). Flowers with anthers at the uninucleate stage, less than 200 µm in anther length, were exposed to 10ºC for 5 days (cold pretreatment) and the anthers were cultured on MS medium supplemented with 2,4-D and/or picloram at various concentrations at 30ºC. The optimal supplement for callus formation was a mixture of 0.075 mg L- 1 2,4-D + 0.075 mg L- 1 picloram or 0.75 mg L- 1 2,4-D without picloram. Only a few calli were induced from the anthers without cold pretreatment. The calli were transplanted to MS medium without phytohormones and cultured at 25ºC for plant regeneration. Among one hundred twenty root tips of the regenerated plantlets examined, 14.2% were haploid (n=8). However, in the plantlets regenerated from anthers without cold pre-treatment only 2.5% was haploid. In both haploid and diploid regenerated plantlets, the chromosome number was fixed without variation. Among the regenerated plantlets, one was albino. Haploid plantlets were transplanted to the field after acclimation in pots filled with vermiculite under 90% humidity for a month, and haploid plant were produced. The potential of haploid plants derived from anther culture for production of high-yield and good-quality cultivars is discussed.