Aaptamine attenuates the proliferation and progression of non-small cell lung carcinoma

• Published in: Pharmaceutical biology Vol. 58; no. 1; pp. 1044 - 1054
• Main Authors: Gong, Kaikai, Miao, Shuang, Yang, Lijuan, Wu, Yan, Guo, Jiwei, Chen, Weiwei, Dai, Juanjuan, Du, Jing, Xi, Sichuan
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01.01.2020; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Apoptosis; Caspase-3; CDK; Cell cycle; Cell proliferation; Cyclin D1; Cyclin-dependent kinase 2; Cylin; G1 phase; Gelatinase B; Invasiveness; Kinases; Lung cancer; Lung carcinoma; Matrilysin; Molecular modelling; Non-small cell lung carcinoma; NSCLC; Phenotypes; PI3K/AKT/GSK3β pathway; Poly(ADP-ribose) polymerase; Signal transduction; Small cell lung carcinoma; Tumorigenesis; Western blotting
• ISSN: 1388-0209; 1744-5116
• DOI: 10.1080/13880209.2020.1822420

Aaptamine is a potent ocean-derived non-traditional drug candidate against human cancers. However, the underlying molecular mechanisms governing aaptamine-mediated repression of lung cancer cells remain largely undefined. To examine the inhibitory effect of aaptamine on proliferation and progression of non-small cell lung carcinoma (NSCLC) and dissect the potential mechanisms involved in its anticancer functions. In vitro assays of cell proliferation, cell cycle analysis, clonal formation, apoptosis and migration were performed to examine the inhibitory effects of aaptamine (8, 16 and 32 μg/mL) on NSCLC cells. The expression levels of proteins were analysed using western blotting analysis when cells were treated with a single drug or a combination treatment for 48 h. Aaptamine significantly inhibited A549 and H1299 cells proliferation with IC 50 values of 13.91 and 10.47 μg/mL. At the concentrations of 16 and 32 μg/mL, aaptamine significantly reduced capacities in clonogenicity, enhanced cellular apoptosis and decreased the motile and invasive cellular phenotype. In addition, aaptamine arrested cell cycle at G1 phase via selectively abating cell cycle regulation drivers (CDK2/4 and Cyclin D1/E). Western blotting results showed that aaptamine attenuated the protein expression of MMP-7, MMP-9 and upregulated the expression of cleaved-PARP and cleaved-caspase 3. Moreover, aaptamine inhibited PI3K/AKT/GSK3β signalling cascades through specifically degrading the phosphorylated AKT and GSK3β. Aaptamine retarded the proliferation and invasion of NSCLC cells by selectively targeting the pathway PI3K/AKT/GSK3β suggesting it as a potential chemotherapeutic agent for repressing tumorigenesis and progression of NSCLC in humans.