Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins

• Published in: Bioscience, biotechnology, and biochemistry Vol. 79; no. 2; pp. 271 - 279
• Main Authors: Nishiyama, Keita, Nakamata, Koichi, Ueno, Shintaro, Terao, Akari, Aryantini, Ni Putu Desy, Sujaya, I. Nengah, Fukuda, Kenji, Urashima, Tadasu, Yamamoto, Yuji, Mukai, Takao
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.02.2015; Oxford University Press
• Subjects: Bacterial Adhesion; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Cell Wall - metabolism; Cloning, Molecular; extracellular matrix (ECM) proteins; Extracellular Matrix Proteins - metabolism; Lactobacillus rhamnosus; Lactobacillus rhamnosus - cytology; Lactobacillus rhamnosus - genetics; Lactobacillus rhamnosus - metabolism; Lactobacillus rhamnosus - physiology; Mucins - metabolism; mucus-binding factor; Mutation; Sequence Analysis; mucus-binding factor; extracellular matrix (ECM) proteins; bacterial adhesion; Lactobacillus rhamnosus
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2014.972325

We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wild-type strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding. Binding of MBF from L. rhamnosus FSMM22 to Mucin and ECM proteins.