The utility of yeast as a tool for cell-based, target-directed high-throughput screening

Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening...

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Published inParasitology Vol. 141; no. 1; pp. 8 - 16
Main Authors NORCLIFFE, J. L., ALVAREZ-RUIZ, E., MARTIN-PLAZA, J. J., STEEL, P. G., DENNY, P. W.
Format Journal Article
LanguageEnglish
Published Cambridge, UK Cambridge University Press 01.01.2014
Subjects
Biological Assay - economics
Biological Assay - utilization
cell culture
Communicable Diseases - drug therapy
cost effectiveness
Drug Delivery Systems
Drug Discovery
Drug Evaluation, Preclinical
Drugs, Investigational - pharmacology
Gene Expression
Genetic Engineering
High-Throughput Screening Assays - economics
High-Throughput Screening Assays - utilization
Humans
Neglected Diseases - drug therapy
Parasites
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
screening
Tropical Medicine
Yeasts
NTDs
HTS
Yeast
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Summary:Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening which takes no account of the target or targets being hit. Despite their successes both of these approaches have limitations; for example, the production of soluble protein and a lack of cellular context or the problems and expense of parasite cell culture. In addition, both can be challenging to miniaturize for ultra (u)HTS and expensive to utilize. Yeast-based systems offer a cost-effective approach to study and screen protein targets in a direct-directed manner within a eukaryotic cellular context. In this review, we examine the utility and limitations of yeast cell-based, target-directed screening. In particular we focus on the currently under-explored possibility of using such formats in uHTS screening campaigns for NTDs.
Bibliography:http://dx.doi.org/10.1017/S0031182013000425
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ISSN:0031-1820
1469-8161
1469-8161
DOI:10.1017/S0031182013000425