Social motility of biofilm-like microcolonies in a gliding bacterium

• Published in: Nature communications Vol. 12; no. 1; p. 5700
• Main Authors: Li, Chao, Hurley, Amanda, Hu, Wei, Warrick, Jay W., Lozano, Gabriel L., Ayuso, Jose M., Pan, Wenxiao, Handelsman, Jo, Beebe, David J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 29.09.2021; Nature Publishing Group UK; Nature Portfolio
• Subjects: Aggregates; Bacteria; Biofilms; Cell surface; Computer simulation; Cores; Flagella; Flavobacterium johnsoniae; Gliding; Microfluidics; Microorganisms; Microscopic analysis; Motility; Plant roots; Polysaccharides; Self-assembly; Solid surfaces; Swarming
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-25408-7

Abstract Bacterial biofilms are aggregates of surface-associated cells embedded in an extracellular polysaccharide (EPS) matrix, and are typically stationary. Studies of bacterial collective movement have largely focused on swarming motility mediated by flagella or pili, in the absence of a biofilm. Here, we describe a unique mode of collective movement by a self-propelled, surface-associated biofilm-like multicellular structure. Flavobacterium johnsoniae cells, which move by gliding motility, self-assemble into spherical microcolonies with EPS cores when observed by an under-oil open microfluidic system. Small microcolonies merge, creating larger ones. Microscopic analysis and computer simulation indicate that microcolonies move by cells at the base of the structure, attached to the surface by one pole of the cell. Biochemical and mutant analyses show that an active process drives microcolony self-assembly and motility, which depend on the bacterial gliding apparatus. We hypothesize that this mode of collective bacterial movement on solid surfaces may play potential roles in biofilm dynamics, bacterial cargo transport, or microbial adaptation. However, whether this collective motility occurs on plant roots or soil particles, the native environment for F. johnsoniae , is unknown.