Effects of a 6-O-Hydroxyethyl Group on the Hydrolysis of 6-O-Hydroxyethylchitin (Glycolchitin) by Chitinase

Kinetic experiments were done on a chitinase from Bacillus sp. with four kinds of 6-O-hydroxyethylchitin (HE-chitin, glycolchitin) with different degrees of substitution (ds) for the 6-O-hydroxyethyl group as substrates. The K m increased markedly by increase in the ds, and the value for HE-chitin w...

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Bibliographic Details
Published inBioscience, biotechnology, and biochemistry Vol. 60; no. 11; pp. 1886 - 1887
Main Authors Inui, Hiroshi, Yoshida, Masashi, Hirano, Shigehiro
Format Journal Article
LanguageEnglish
Published Tokyo Taylor & Francis 1996
Japan Society for Bioscience Biotechnology and Agrochemistry
Oxford University Press
Subjects
6-O-substitution
Analytical, structural and metabolic biochemistry
Bacteriology
Biological and medical sciences
chitin
chitinase
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
glycolchitin
Hydrolases
kinetics
Metabolism. Enzymes
Microbiology
Chitinase
Substituent effect
Enzyme
Bacillaceae
Hydrolysis
Bacillales
Enzymatic activity
Glycosidases
Bacillus
Bacteria
Hydrolases
Hydroxyethylation
Kinetic parameter
O-Glycosidases
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Summary:Kinetic experiments were done on a chitinase from Bacillus sp. with four kinds of 6-O-hydroxyethylchitin (HE-chitin, glycolchitin) with different degrees of substitution (ds) for the 6-O-hydroxyethyl group as substrates. The K m increased markedly by increase in the ds, and the value for HE-chitin with the ds of 1.0 was 4.5-fold higher than that for a substrate with the ds of 0.33. In contrast, the V max decreased in response to an increase in the ds, and the value for the former substrate was about a half of that for the latter one.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60.1886