3D high spatial resolution visualisation and quantification of interconnectivity in polymer films

• Published in: International journal of pharmaceutics Vol. 587; p. 119622
• Main Authors: Fager, C., Barman, S., Röding, M., Olsson, A., Lorén, N., von Corswant, C., Bolin, D., Rootzén, H., Olsson, E.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.09.2020
• Subjects: beam; Bottlenecks; cellulose; connectivity; controlled drug release; data visualization; diffusion; electron beam; ethyl cellulose; Focused ion; Focused ion beam; focused ion beam scanning electron microscopy tomography; Geodesic channels; Geodesic paths; hydroxypropylcellulose; image segmentation; Interconnectivity; Matematik; Mathematics; microstructure; permeability; Pharmacology & Pharmacy; Polymer; pore structure; Porosity; porous polymer; priority journal; quantitative analysis; Scanning electron microscopy; three-dimensional imaging; tomography; tortuosity; Visualisation; 3D; Scanning electron microscopy; Interconnectivity; Geodesic channels; Focused ion beam; Visualisation; Polymer; Geodesic paths; Porosity; Bottlenecks
• ISSN: 0378-5173; 1873-3476; 1873-3476
• DOI: 10.1016/j.ijpharm.2020.119622

[Display omitted] A porous network acts as transport paths for drugs through films for controlled drug release. The interconnectivity of the network strongly influences the transport properties. It is therefore important to quantify the interconnectivity and correlate it to transport properties for control and design of new films. This work presents a novel method for 3D visualisation and analysis of interconnectivity. High spatial resolution 3D data on porous polymer films for controlled drug release has been acquired using a focused ion beam (FIB) combined with a scanning electron microscope (SEM). The data analysis method enables visualisation of pore paths starting at a chosen inlet pore, dividing them into groups by length, enabling a more detailed quantification and visualisation. The method also enables identification of central features of the porous network by quantification of channels where pore paths coincide. The method was applied to FIB-SEM data of three leached ethyl cellulose (EC)/hydroxypropyl cellulose (HPC) films with different weight percentages. The results from the analysis were consistent with the experimentally measured release properties of the films. The interconnectivity and porosity increase with increasing amount of HPC. The bottleneck effect was strong in the leached film with lowest porosity.