Network Context and Selection in the Evolution to Enzyme Specificity
Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However,...
Saved in:
| Published in | Science (American Association for the Advancement of Science) Vol. 337; no. 6098; pp. 1101 - 1104 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Association for the Advancement of Science
31.08.2012
The American Association for the Advancement of Science |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However, it is not apparent why these generalist enzymes remain. Here, we show that there are marked differences between generalist enzymes anf specialist enzymes, known to catalyze a single chemical reaction on one particular substrate in vivo. Specialist enzymes (i) are frequently essential, (ii) maintain higher metabolic flux, and (iii) require more regulation of enzyme activity to control metabolic flux in dynamic environments than do generalist enzymes. Furthermore, these properties are conserved in Archaea and Eukarya. Thus, the metabolic network context and environmental conditions influence enzyme evolution toward high specificity. |
|---|---|
| AbstractList | Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However, it is not apparent why these generalist enzymes remain. Here, we show that there are marked differences between generalist enzymes and specialist enzymes, known to catalyze a single chemical reaction on one particular substrate in vivo. Specialist enzymes (i) are frequently essential, (ii) maintain higher metabolic flux, and (iii) require more regulation of enzyme activity to control metabolic flux in dynamic environments than do generalist enzymes. Furthermore, these properties are conserved in Archaea and Eukarya. Thus, the metabolic network context and environmental conditions influence enzyme evolution toward high specificity. Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However, it is not apparent why these generalist enzymes remain. Here, we show that there are marked differences between generalist enzymes anf specialist enzymes, known to catalyze a single chemical reaction on one particular substrate in vivo. Specialist enzymes (i) are frequently essential, (ii) maintain higher metabolic flux, and (iii) require more regulation of enzyme activity to control metabolic flux in dynamic environments than do generalist enzymes. Furthermore, these properties are conserved in Archaea and Eukarya. Thus, the metabolic network context and environmental conditions influence enzyme evolution toward high specificity. Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However, it is not apparent why these generalist enzymes remain. Here, we show that there are marked differences between generalist enzymes and specialist enzymes, known to catalyze a single chemical reaction on one particular substrate in vivo. Specialist enzymes (i) are frequently essential, (ii) maintain higher metabolic flux, and (iii) require more regulation of enzyme activity to control metabolic flux in dynamic environments than do generalist enzymes. Furthermore, these properties are conserved in Archaea and Eukarya. Thus, the metabolic network context and environmental conditions influence enzyme evolution toward high specificity. Good Enough Can Be Good Enough To begin to understand why some enzymes are promiscuous and have many substrates, whereas others are highly specific, and why some have high activity, whereas others appear not to be optimized, Nam et al. (p. 1101 ) analyzed metabolic networks in bacteria. Specialist enzymes are essential for life, catalyze a high flux of enzymatic activity, and are more highly regulated. However, not all enzymes appear to be on a track of gradual improvement of specificity and efficiency. Generalist enzymes seem to well serve their own purposes, and their optimization may not justify the evolutionary cost. Are less promiscuous enzymes more highly evolved? Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However, it is not apparent why these generalist enzymes remain. Here, we show that there are marked differences between generalist enzymes and specialist enzymes, known to catalyze a single chemical reaction on one particular substrate in vivo. Specialist enzymes (i) are frequently essential, (ii) maintain higher metabolic flux, and (iii) require more regulation of enzyme activity to control metabolic flux in dynamic environments than do generalist enzymes. Furthermore, these properties are conserved in Archaea and Eukarya. Thus, the metabolic network context and environmental conditions influence enzyme evolution toward high specificity. To begin to understand why some enzymes are promiscuous and have many substrates, whereas others are highly specific, and why some have high activity, whereas others appear not to be optimized, Nam et al. (p. 1101) analyzed metabolic networks in bacteria. Specialist enzymes are essential for life, catalyze a high flux of enzymatic activity, and are more highly regulated. However, not all enzymes appear to be on a track of gradual improvement of specificity and efficiency. Generalist enzymes seem to well serve their own purposes, and their optimization may not justify the evolutionary cost. [PUBLICATION ABSTRACT] Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However, it is not apparent why these generalist enzymes remain. Here, we show that there are marked differences between generalist enzymes and specialist enzymes, known to catalyze a single chemical reaction on one particular substrate in vivo. Specialist enzymes (i) are frequently essential, (ii) maintain higher metabolic flux, and (iii) require more regulation of enzyme activity to control metabolic flux in dynamic environments than do generalist enzymes. Furthermore, these properties are conserved in Archaea and Eukarya. Thus, the metabolic network context and environmental conditions influence enzyme evolution toward high specificity. [PUBLICATION ABSTRACT] |
| Author | Lerman, Joshua A. Nam, Hojung Lewis, Nathan E. Chang, Roger L. Kim, Donghyuk Palsson, Bernhard O. Lee, Dae-Hee |
| AuthorAffiliation | 1 Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093-0412, USA 2 Bioinformatics and Systems Biology Graduate Program, University of California San Diego, La Jolla, CA, 92093-0412, USA 3 Wyss Institute for Biologically Inspired Engineering and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA |
| AuthorAffiliation_xml | – name: 2 Bioinformatics and Systems Biology Graduate Program, University of California San Diego, La Jolla, CA, 92093-0412, USA – name: 3 Wyss Institute for Biologically Inspired Engineering and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA – name: 1 Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093-0412, USA |
| Author_xml | – sequence: 1 givenname: Hojung surname: Nam fullname: Nam, Hojung – sequence: 2 givenname: Nathan E. surname: Lewis fullname: Lewis, Nathan E. – sequence: 3 givenname: Joshua A. surname: Lerman fullname: Lerman, Joshua A. – sequence: 4 givenname: Dae-Hee surname: Lee fullname: Lee, Dae-Hee – sequence: 5 givenname: Roger L. surname: Chang fullname: Chang, Roger L. – sequence: 6 givenname: Donghyuk surname: Kim fullname: Kim, Donghyuk – sequence: 7 givenname: Bernhard O. surname: Palsson fullname: Palsson, Bernhard O. |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26369425$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/22936779$$D View this record in MEDLINE/PubMed |
| BookMark | eNpdkc1vEzEQxS1URNPAmRNoJYTEZVvbE9vrCxJKw4dUwaFwtmyvlzps7NT2loa_nk2ztMBppHm_eXqjd4KOQgwOoecEnxJC-Vm23gXrTgklvOHkEZoRLFktKYYjNMMYeN1gwY7RSc5rjEdNwhN0TKkELoScofPPrvyM6Ue1jKG421Lp0FaXrne2-BgqH6py5arVTeyHu0WJ1Sr82m1cdbl11nfe-rJ7ih53us_u2TTn6Nv71dflx_riy4dPy3cXtWUgS60l5gKs5KYhFhthaeswNy0zVBIDgnTQUi0bgH1ww5q2XWhMxKLlXWeMgTl6e_DdDmbjWutCSbpX2-Q3Ou1U1F79qwR_pb7HGwUMOOZ8NHgzGaR4Pbhc1MZn6_peBxeHrAgGwRkXbI---g9dxyGF8b07CghdjEHn6OxA2RRzTq67D0Ow2jekpobU1NB48fLvH-75P5WMwOsJ0Nnqvks6WJ8fOA5cLigbuRcHbp1LTA86UN5IQeE3qLmmMQ |
| CODEN | SCIEAS |
| CitedBy_id | crossref_primary_10_1038_s41598_017_09581_8 crossref_primary_10_4236_ajor_2013_31A007 crossref_primary_10_1038_s41929_022_00798_z crossref_primary_10_1038_ncomms8534 crossref_primary_10_3389_fmicb_2016_00530 crossref_primary_10_1111_jfpp_14370 crossref_primary_10_1186_s12934_022_01933_y crossref_primary_10_1093_femsle_fny241 crossref_primary_10_1042_BCJ20160699 crossref_primary_10_1186_s13068_017_0900_3 crossref_primary_10_1186_s13321_015_0087_1 crossref_primary_10_1016_j_tibtech_2015_12_005 crossref_primary_10_1038_srep03925 crossref_primary_10_1371_journal_pcbi_1003554 crossref_primary_10_3390_ijms24108734 crossref_primary_10_1002_prot_24847 crossref_primary_10_1371_journal_pcbi_1008208 crossref_primary_10_1016_j_biotechadv_2023_108119 crossref_primary_10_1007_s12298_022_01188_2 crossref_primary_10_1021_acssynbio_9b00447 crossref_primary_10_15252_msb_20145307 crossref_primary_10_3389_fpls_2017_00613 crossref_primary_10_1002_jez_b_22562 crossref_primary_10_1016_j_scib_2023_08_056 crossref_primary_10_1371_journal_pcbi_1005895 crossref_primary_10_1016_j_pbi_2014_02_010 crossref_primary_10_15252_msb_202110427 crossref_primary_10_1038_s41540_020_00151_9 crossref_primary_10_7554_eLife_76757 crossref_primary_10_1098_rsif_2013_1190 crossref_primary_10_1002_bit_25860 crossref_primary_10_1016_j_biosystems_2015_12_004 crossref_primary_10_1134_S0003683817050131 crossref_primary_10_3389_fevo_2020_564071 crossref_primary_10_1186_s40508_016_0047_8 crossref_primary_10_1021_acssynbio_9b00318 crossref_primary_10_1038_embor_2013_178 crossref_primary_10_1016_j_gde_2022_101966 crossref_primary_10_1007_s00049_020_00322_4 crossref_primary_10_1039_C5RA23949D crossref_primary_10_1016_j_cell_2017_12_006 crossref_primary_10_1016_j_cell_2015_05_019 crossref_primary_10_1088_1478_3975_10_1_011001 crossref_primary_10_1016_j_biotechadv_2016_11_004 crossref_primary_10_1002_anie_201300766 crossref_primary_10_3390_life7030030 crossref_primary_10_1016_j_jsb_2022_107835 crossref_primary_10_1073_pnas_1423664112 crossref_primary_10_1098_rspb_2016_1536 crossref_primary_10_1186_1752_0509_8_48 crossref_primary_10_1093_nar_gkaa446 crossref_primary_10_1002_biot_201200279 crossref_primary_10_1073_pnas_2405524121 crossref_primary_10_1039_C4CS00351A crossref_primary_10_1016_j_copbio_2012_10_006 crossref_primary_10_1073_pnas_1414218112 crossref_primary_10_1093_gbe_evv217 crossref_primary_10_1111_acel_13595 crossref_primary_10_1038_nature12301 crossref_primary_10_1093_bib_bbz065 crossref_primary_10_1016_j_coisb_2022_100417 crossref_primary_10_1093_pcp_pct203 crossref_primary_10_1111_1462_2920_12270 crossref_primary_10_1074_jbc_M114_595363 crossref_primary_10_3390_molecules26216745 crossref_primary_10_1016_j_ymben_2021_03_013 crossref_primary_10_1021_acssynbio_9b00419 crossref_primary_10_1038_s41467_018_07652_6 crossref_primary_10_1098_rspb_2015_2572 crossref_primary_10_1074_jbc_M112_427922 crossref_primary_10_1371_journal_pcbi_1008647 crossref_primary_10_1126_science_adf2465 crossref_primary_10_1021_ci400368v crossref_primary_10_1371_journal_pcbi_1005140 crossref_primary_10_1039_D3CC04209J crossref_primary_10_1016_j_csbj_2020_11_050 crossref_primary_10_1186_s12915_014_0110_4 crossref_primary_10_1002_bit_25133 crossref_primary_10_1016_j_biortech_2019_121730 crossref_primary_10_1038_s41467_019_09579_y crossref_primary_10_1186_s12918_016_0271_6 crossref_primary_10_1186_1752_0509_8_64 crossref_primary_10_1021_acscatal_3c04461 crossref_primary_10_1002_ajb2_1216 crossref_primary_10_1016_j_coisb_2019_04_004 crossref_primary_10_1371_journal_pone_0069707 crossref_primary_10_1021_acs_biochem_6b00887 crossref_primary_10_1111_1574_6968_12109 crossref_primary_10_1016_j_cej_2022_140470 crossref_primary_10_1021_jacsau_2c00344 crossref_primary_10_1042_BST20200239 crossref_primary_10_1016_j_soilbio_2014_08_021 crossref_primary_10_1038_s41467_023_40455_y crossref_primary_10_1186_s12918_016_0343_7 crossref_primary_10_1016_j_coisb_2021_100379 crossref_primary_10_1093_jxb_ers297 crossref_primary_10_1021_acscatal_9b01031 crossref_primary_10_1371_journal_pone_0208782 crossref_primary_10_1016_j_bmc_2013_04_052 crossref_primary_10_1007_s00018_014_1643_y crossref_primary_10_1093_bioinformatics_btu760 crossref_primary_10_1016_j_plantsci_2018_03_025 crossref_primary_10_3390_metabo11090606 crossref_primary_10_1098_rsif_2014_0419 crossref_primary_10_1016_j_rser_2021_112025 crossref_primary_10_1042_BJ20141493 crossref_primary_10_1016_j_jsb_2015_04_009 crossref_primary_10_1021_sb4001273 crossref_primary_10_1371_journal_pone_0087950 crossref_primary_10_3389_fmicb_2015_00958 crossref_primary_10_1016_j_xplc_2019_100012 crossref_primary_10_1002_ange_201300766 crossref_primary_10_1111_mmi_12801 crossref_primary_10_1080_10242422_2019_1696779 crossref_primary_10_1111_1462_2920_13950 crossref_primary_10_1093_icb_icw026 crossref_primary_10_1371_journal_pcbi_1003837 crossref_primary_10_1007_s00018_022_04579_6 crossref_primary_10_1128_mBio_02615_21 crossref_primary_10_1016_j_ymben_2017_12_002 crossref_primary_10_1093_gbe_evac114 crossref_primary_10_1096_fj_12_224014 crossref_primary_10_1080_21655979_2015_1075674 crossref_primary_10_1016_j_biotechadv_2022_108015 crossref_primary_10_1007_s11705_017_1628_0 crossref_primary_10_1038_s41598_020_75772_5 crossref_primary_10_3389_fcell_2020_00451 crossref_primary_10_1186_s12862_018_1164_7 crossref_primary_10_1371_journal_pone_0112464 crossref_primary_10_1016_j_ymben_2014_11_005 crossref_primary_10_3389_fenvs_2016_00036 crossref_primary_10_1371_journal_pone_0061348 crossref_primary_10_1016_j_ymben_2020_07_003 crossref_primary_10_1038_srep16025 crossref_primary_10_1093_nar_gky069 crossref_primary_10_1038_s42003_021_02867_8 crossref_primary_10_1021_acs_biochem_6b00723 crossref_primary_10_1093_nar_gkaa230 crossref_primary_10_1093_nar_gkz030 crossref_primary_10_1089_ind_2013_0008 crossref_primary_10_3390_ijms242216194 crossref_primary_10_1371_journal_pcbi_1004705 crossref_primary_10_1111_lam_12428 crossref_primary_10_15252_msb_20188462 crossref_primary_10_1093_molbev_msac217 crossref_primary_10_3390_metabo10040160 crossref_primary_10_1007_s00792_012_0509_1 crossref_primary_10_1042_BCJ20160289 crossref_primary_10_1016_j_micres_2016_10_006 crossref_primary_10_1093_bib_bby022 crossref_primary_10_1039_c3np70045c crossref_primary_10_1016_j_celrep_2017_07_015 crossref_primary_10_1016_j_synbio_2017_11_002 crossref_primary_10_1093_femsle_fnad027 crossref_primary_10_1128_AEM_01709_16 crossref_primary_10_3390_jof7121020 crossref_primary_10_1021_acs_chemrev_8b00753 crossref_primary_10_1002_btpr_2233 crossref_primary_10_1016_j_gene_2015_02_032 crossref_primary_10_1016_j_copbio_2015_10_008 crossref_primary_10_3389_fchem_2017_00041 crossref_primary_10_1021_acsinfecdis_2c00402 crossref_primary_10_15252_msb_20145243 crossref_primary_10_1016_j_scitotenv_2021_151226 crossref_primary_10_1111_1462_2920_14843 crossref_primary_10_1021_acssynbio_5b00294 crossref_primary_10_1016_j_tree_2016_10_017 crossref_primary_10_1093_molbev_msad202 crossref_primary_10_1002_iub_1178 crossref_primary_10_1021_cr500628b crossref_primary_10_1371_journal_pcbi_1004329 crossref_primary_10_1038_ncomms2246 crossref_primary_10_1038_s41467_017_02362_x crossref_primary_10_1038_nrg3643 crossref_primary_10_1016_j_biosystems_2023_105031 crossref_primary_10_1038_nmeth_4103 crossref_primary_10_1146_annurev_biochem_062917_012023 crossref_primary_10_3390_molecules26185693 crossref_primary_10_1016_j_micres_2014_02_007 crossref_primary_10_1111_evo_13487 crossref_primary_10_1186_1752_0509_9_S3_S4 crossref_primary_10_3390_molecules23113008 crossref_primary_10_1016_j_ymben_2015_11_006 crossref_primary_10_1002_bit_27714 crossref_primary_10_1016_j_cbpa_2014_05_008 crossref_primary_10_1016_j_tibs_2018_09_015 crossref_primary_10_1042_BST20170272 crossref_primary_10_1073_pnas_1508573112 crossref_primary_10_1016_j_abb_2012_11_011 crossref_primary_10_1074_jbc_R114_572990 crossref_primary_10_1038_srep10008 crossref_primary_10_1016_j_coph_2013_05_006 crossref_primary_10_1016_j_pharmthera_2013_01_016 crossref_primary_10_1016_j_tibtech_2018_08_001 crossref_primary_10_1038_s41467_021_22022_5 crossref_primary_10_1038_nchembio_1090 crossref_primary_10_1039_c5pp00245a crossref_primary_10_1073_pnas_1406102111 crossref_primary_10_1371_journal_pcbi_1007185 crossref_primary_10_1111_febs_13274 |
| Cites_doi | 10.1128/jb.163.1.1-7.1985 10.1074/jbc.R800048200 10.1038/nbt.1711 10.1021/bi2002289 10.1186/1752-0509-3-37 10.1093/nar/gkq1143 10.1038/msb.2010.47 10.1074/jbc.M111.274050 10.1038/nrg3033 10.1038/nrg2689 10.1074/jbc.M309578200 10.1101/gr.3832305 10.1038/msb4100046 10.1371/journal.pcbi.1001050 10.1038/msb4100155 10.1074/jbc.271.44.27795 10.1006/jtbi.1993.1202 10.1016/j.jtbi.2007.12.008 10.1128/JB.00034-09 10.1128/JB.185.9.2692-2699.2003 10.1002/jez.b.21152 10.1002/(SICI)1097-0290(19971120)56:4<398::AID-BIT6>3.0.CO;2-J 10.1046/j.0014-2956.2001.02606.x 10.1016/S0168-6445(05)80006-3 10.1016/j.bpj.2010.12.3707 10.1038/nrmicro2669 10.1074/jbc.R111.254714 10.1016/j.copbio.2011.04.016 10.1128/jb.118.1.83-88.1974 10.1021/bi101763c 10.1002/bit.260350711 10.1146/annurev-genet-102209-163517 10.1038/nature02456 10.1016/S0021-9258(17)35791-5 10.1038/nrmicro2737 10.1038/nbt.1614 10.1016/S0006-3495(02)75150-3 10.1146/annurev.mi.30.100176.002205 10.1038/nature08112 10.1046/j.1365-2958.1998.00696.x 10.1038/msb.2011.65 10.1038/ng1856 10.1038/msb4100162 10.1038/msb.2010.68 10.1371/journal.pcbi.0010068 10.1128/AEM.00373-10 10.1016/j.mib.2010.03.003 10.1093/nar/gkp896 10.1038/msb4100050 10.1038/ng1482 10.1038/msb.2011.52 10.1007/978-3-642-86659-3 10.1371/journal.pcbi.1000859 10.1038/nature01149 10.1016/j.copbio.2011.03.006 10.1073/pnas.0907176107 10.1126/science.1174671 10.1038/nbt1401 10.1128/jb.170.11.5330-5336.1988 10.1038/msb.2011.42 10.1074/mcp.M700311-MCP200 10.1371/journal.pcbi.1000938 10.1038/nature02636 10.1016/j.ymben.2003.09.002 10.1038/nchembio.495 10.1126/science.1188308 10.1146/annurev-biochem-030409-143718 10.1371/journal.pcbi.1000312 10.1038/nature02289 10.1074/mcp.M800187-MCP200 10.1073/pnas.97.10.5528 10.1038/nprot.2011.308 10.1038/nchembio.186 10.1016/S0021-9258(18)77290-6 10.1038/nrg2808 |
| ContentType | Journal Article |
| Copyright | Copyright © 2012 American Association for the Advancement of Science 2014 INIST-CNRS Copyright © 2012, American Association for the Advancement of Science |
| Copyright_xml | – notice: Copyright © 2012 American Association for the Advancement of Science – notice: 2014 INIST-CNRS – notice: Copyright © 2012, American Association for the Advancement of Science |
| DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QF 7QG 7QL 7QP 7QQ 7QR 7SC 7SE 7SN 7SP 7SR 7SS 7T7 7TA 7TB 7TK 7TM 7U5 7U9 8BQ 8FD C1K F28 FR3 H8D H8G H94 JG9 JQ2 K9. KR7 L7M L~C L~D M7N P64 RC3 7X8 5PM |
| DOI | 10.1126/science.1216861 |
| DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Aluminium Industry Abstracts Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Ceramic Abstracts Chemoreception Abstracts Computer and Information Systems Abstracts Corrosion Abstracts Ecology Abstracts Electronics & Communications Abstracts Engineered Materials Abstracts Entomology Abstracts (Full archive) Industrial and Applied Microbiology Abstracts (Microbiology A) Materials Business File Mechanical & Transportation Engineering Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Solid State and Superconductivity Abstracts Virology and AIDS Abstracts METADEX Technology Research Database Environmental Sciences and Pollution Management ANTE: Abstracts in New Technology & Engineering Engineering Research Database Aerospace Database Copper Technical Reference Library AIDS and Cancer Research Abstracts Materials Research Database ProQuest Computer Science Collection ProQuest Health & Medical Complete (Alumni) Civil Engineering Abstracts Advanced Technologies Database with Aerospace Computer and Information Systems Abstracts Academic Computer and Information Systems Abstracts Professional Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Materials Research Database Technology Research Database Computer and Information Systems Abstracts – Academic Mechanical & Transportation Engineering Abstracts Nucleic Acids Abstracts ProQuest Computer Science Collection Computer and Information Systems Abstracts ProQuest Health & Medical Complete (Alumni) Materials Business File Environmental Sciences and Pollution Management Aerospace Database Copper Technical Reference Library Engineered Materials Abstracts Genetics Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Advanced Technologies Database with Aerospace ANTE: Abstracts in New Technology & Engineering Civil Engineering Abstracts Aluminium Industry Abstracts Virology and AIDS Abstracts Electronics & Communications Abstracts Ceramic Abstracts Ecology Abstracts Neurosciences Abstracts METADEX Biotechnology and BioEngineering Abstracts Computer and Information Systems Abstracts Professional Entomology Abstracts Animal Behavior Abstracts Solid State and Superconductivity Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts Corrosion Abstracts MEDLINE - Academic |
| DatabaseTitleList | MEDLINE CrossRef Materials Research Database |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Biology Chemistry |
| EISSN | 1095-9203 |
| EndPage | 1104 |
| ExternalDocumentID | 2748984501 10_1126_science_1216861 22936779 26369425 23268972 |
| Genre | Research Support, U.S. Gov't, Non-P.H.S Journal Article Research Support, N.I.H., Extramural Feature |
| GrantInformation_xml | – fundername: NIGMS NIH HHS grantid: 2R01GM057089-13 – fundername: NIGMS NIH HHS grantid: R01 GM057089 – fundername: National Institute of General Medical Sciences : NIGMS grantid: R01 GM057089 || GM |
| GroupedDBID | --- --Z -DZ -ET -~X .-4 ..I .55 .DC .HR 08G 0B8 0R~ 0WA 123 18M 2FS 2KS 2WC 34G 36B 39C 3R3 4.4 4R4 53G 5RE 63O 68V 6OB 6TJ 7X2 7~K 85S 8F7 AABCJ AACGO AAIKC AAJYS AAMNW AANCE AAWTO AAYJJ ABBHK ABCQX ABDBF ABDEX ABEFU ABIVO ABOCM ABPLY ABPMR ABPPZ ABQIJ ABTLG ABWJO ABXSQ ABZEH ACBEA ACBEC ACGFO ACGFS ACGOD ACIWK ACMJI ACNCT ACPRK ACQAM ACQOY ACTDY ADDRP ADULT ADZLD AEGBM AENEX AETEA AEUPB AEXZC AFCHL AFDAS AFFDN AFFNX AFHKK AFOSN AFQFN AFRAH AGCDD AGFXO AGNAY AGSOS AHMBA AHPSJ AIDAL AIDUJ AJGZS ALMA_UNASSIGNED_HOLDINGS ANJGP AQVQM ASPBG AVWKF B-7 BKF BLC C2- C45 CS3 DB2 DCCCD DNJUQ DOOOF DU5 DWIUU EBS EJD EMOBN ESX F5P FA8 FEDTE GX1 HZ~ I.T IAO IEA IGG IGS IH2 IHR INH INR IOF IOV IPO IPY ISE J9C JAAYA JBMMH JCF JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST K-O KCC L7B LSO LU7 M0P MQT MVM N9A NEJ NHB O9- OCB OFXIZ OGEVE OK1 OMK OVD P-O P2P PQQKQ PZZ QJJ RHF RHI RXW SA0 SC5 SJN TAE TEORI TN5 TWZ UBW UCV UHB UKR UMD UNMZH UQL USG VQA VVN WH7 WI4 X7M XFK XJF XZL Y6R YCJ YK4 YKV YNT YOJ YR2 YRY YSQ YV5 YWH YYP YYQ YZZ ZA5 ZCA ZE2 ~02 ~H1 ~KM ~ZZ .GJ .GO 0-V 08R 186 2XV 3EH 3V. 41~ 42X 66. 692 6XO 79B 7X7 7XC 88A 88E 88I 8AF 8CJ 8FE 8FG 8FH 8FI 8FJ 8G5 8GL 8W4 8WZ 97F A6W AADHG AAFWJ AAKAS AALRV AAYOK ABFLS ABJCF ABPTK ABQIS ABTAH ABUWG ADBBV ADGIM ADZCM AFKRA AJUXI ALSLI ARALO ARAPS ATCPS AZQEC B4K BBAFP BBNVY BBWZM BCU BEC BENPR BGLVJ BHPHI BKNYI BKSAR BPHCQ BVXVI CJNVE D0S D1I D1J D1K DWQXO D~A EAU EGS ESL EWM EX3 F20 FYUFA G8K GICCO GNUQQ GUQSH HCIFZ HGD HQ3 HTVGU HVGLF IAG IBG IEP IER IPC IQODW ISN ISR ITC J5H K6- K9- KB. KQ8 L6V LK5 LK8 M0K M0L M0R M1P M2O M2P M2Q M7P M7R M7S N4W P62 PATMY PCBAR PDBOC PK8 PQEST PQUKI PROAC PSQYO PTHSS PV9 PYCSY QS- R05 RNS RZL SJFOW SKT UBY UHU UKHRP UMP VOH WOQ WOW X7L XKJ XOL YJ6 YXB ZCF ZCG ZGI ZKG ZVL ZVM ZXP ZY4 ~G0 ADACV ADUKH AFRQD ALIPV CGR CUY CVF ECM EIF IPSME NPM UIG AAYXX CITATION 7QF 7QG 7QL 7QP 7QQ 7QR 7SC 7SE 7SN 7SP 7SR 7SS 7T7 7TA 7TB 7TK 7TM 7U5 7U9 8BQ 8FD C1K F28 FR3 H8D H8G H94 JG9 JQ2 K9. KR7 L7M L~C L~D M7N P64 RC3 7X8 5PM |
| ID | FETCH-LOGICAL-c539t-a90673c96b81c0b7c2de06bd5b291b371f3d2a98330036b58dd4a0174d6ffbbb3 |
| ISSN | 0036-8075 |
| IngestDate | Tue Sep 17 21:19:03 EDT 2024 Sat Aug 17 01:02:18 EDT 2024 Fri Sep 13 00:59:08 EDT 2024 Fri Aug 23 03:35:32 EDT 2024 Tue Aug 27 13:46:44 EDT 2024 Fri Nov 25 01:07:57 EST 2022 Fri Feb 02 07:03:09 EST 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6098 |
| Keywords | Molecular evolution Specificity Enzyme Escherichia coli Selection Bacteria Metabolism Gram negative bacteria Enterobacteriaceae |
| Language | English |
| License | CC BY 4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c539t-a90673c96b81c0b7c2de06bd5b291b371f3d2a98330036b58dd4a0174d6ffbbb3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Current address: Systems and Synthetic Biology Research Center. Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea The first two authors contributed equally. |
| OpenAccessLink | https://europepmc.org/articles/pmc3536066?pdf=render |
| PMID | 22936779 |
| PQID | 1037312483 |
| PQPubID | 1256 |
| PageCount | 4 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_3536066 proquest_miscellaneous_1037656756 proquest_journals_1037312483 crossref_primary_10_1126_science_1216861 pubmed_primary_22936779 pascalfrancis_primary_26369425 jstor_primary_23268972 |
| PublicationCentury | 2000 |
| PublicationDate | 2012-08-31 |
| PublicationDateYYYYMMDD | 2012-08-31 |
| PublicationDate_xml | – month: 08 year: 2012 text: 2012-08-31 day: 31 |
| PublicationDecade | 2010 |
| PublicationPlace | Washington, DC |
| PublicationPlace_xml | – name: Washington, DC – name: United States – name: Washington |
| PublicationTitle | Science (American Association for the Advancement of Science) |
| PublicationTitleAlternate | Science |
| PublicationYear | 2012 |
| Publisher | American Association for the Advancement of Science The American Association for the Advancement of Science |
| Publisher_xml | – name: American Association for the Advancement of Science – name: The American Association for the Advancement of Science |
| References | 21600757 - Curr Opin Biotechnol. 2011 Aug;22(4):566-75 20235827 - Annu Rev Biochem. 2010;79:471-505 20657658 - PLoS Comput Biol. 2010;6(7):e1000859 21808261 - Nat Rev Genet. 2011 Sep;12(9):591-602 17625511 - Mol Syst Biol. 2007;3:119 19363119 - J Bacteriol. 2009 Jun;191(11):3437-44 21151122 - Nat Chem Biol. 2011 Jan;7(1):58-63 22020261 - Nat Rev Microbiol. 2011 Dec;9(12):839-48 16941010 - Nat Genet. 2006 Sep;38(9):993-8 17262826 - J Exp Zool B Mol Dev Evol. 2007 May 15;308(3):322-4 791073 - Annu Rev Microbiol. 1976;30:409-25 21811229 - Mol Syst Biol. 2011;7:518 15568024 - Nat Genet. 2005 Jan;37(1):73-6 20051986 - Nat Rev Genet. 2010 Feb;11(2):97-108 15190353 - Nature. 2004 Jun 10;429(6992):661-4 19321003 - BMC Syst Biol. 2009;3:37 21481583 - Curr Opin Biotechnol. 2011 Aug;22(4):595-600 22052908 - J Biol Chem. 2011 Dec 23;286(51):43994-4004 14985762 - Nature. 2004 Feb 26;427(6977):839-43 22069330 - J Biol Chem. 2012 Jan 2;287(1):3-10 16738551 - Mol Syst Biol. 2006;2:2006.0004 22367118 - Nat Rev Microbiol. 2012 Apr;10(4):291-305 17593909 - Mol Syst Biol. 2007;3:121 21332126 - Biochemistry. 2011 Apr 5;50(13):2683-90 16738554 - Mol Syst Biol. 2006;2:2006.0008 16362071 - PLoS Comput Biol. 2005 Dec;1(7):e68 21506553 - Biochemistry. 2011 May 31;50(21):4402-10 20822442 - Annu Rev Genet. 2010;44:25-51 e_1_3_2_26_2 e_1_3_2_49_2 Lewis N. E. (e_1_3_2_8_2) 2012; 10 e_1_3_2_28_2 Gur E. (e_1_3_2_17_2) 2011; 9 e_1_3_2_41_2 e_1_3_2_64_2 e_1_3_2_20_2 e_1_3_2_43_2 e_1_3_2_22_2 e_1_3_2_45_2 e_1_3_2_24_2 e_1_3_2_66_2 Khersonsky O. (e_1_3_2_50_2) 2010; 79 Hopkins S. (e_1_3_2_70_2) 1986; 261 e_1_3_2_60_2 Schellenberger J. (e_1_3_2_40_2) 2011; 6 Sawers G. (e_1_3_2_74_2) 1988; 170 e_1_3_2_9_2 e_1_3_2_16_2 e_1_3_2_37_2 e_1_3_2_7_2 e_1_3_2_18_2 e_1_3_2_39_2 e_1_3_2_54_2 e_1_3_2_75_2 e_1_3_2_31_2 e_1_3_2_52_2 e_1_3_2_73_2 e_1_3_2_5_2 e_1_3_2_12_2 e_1_3_2_33_2 e_1_3_2_58_2 e_1_3_2_79_2 e_1_3_2_3_2 e_1_3_2_14_2 e_1_3_2_35_2 e_1_3_2_56_2 e_1_3_2_77_2 e_1_3_2_71_2 Stover P. (e_1_3_2_68_2) 1990; 265 Schirch V. (e_1_3_2_69_2) 1985; 163 e_1_3_2_27_2 e_1_3_2_48_2 e_1_3_2_29_2 e_1_3_2_65_2 e_1_3_2_21_2 e_1_3_2_42_2 e_1_3_2_63_2 e_1_3_2_23_2 e_1_3_2_44_2 Yu B. J. (e_1_3_2_47_2) 2008; 18 e_1_3_2_25_2 e_1_3_2_46_2 e_1_3_2_67_2 e_1_3_2_61_2 e_1_3_2_15_2 e_1_3_2_38_2 e_1_3_2_59_2 e_1_3_2_6_2 e_1_3_2_19_2 e_1_3_2_30_2 e_1_3_2_53_2 e_1_3_2_76_2 e_1_3_2_32_2 e_1_3_2_51_2 e_1_3_2_11_2 e_1_3_2_34_2 e_1_3_2_57_2 e_1_3_2_4_2 e_1_3_2_13_2 e_1_3_2_36_2 e_1_3_2_55_2 e_1_3_2_78_2 e_1_3_2_2_2 e_1_3_2_72_2 Cocks G. T. (e_1_3_2_62_2) 1974; 118 |
| References_xml | – volume: 163 start-page: 1 year: 1985 ident: e_1_3_2_69_2 article-title: Serine hydroxymethyltransferase from Escherichia coli: Purification and properties publication-title: J. Bacteriol. doi: 10.1128/jb.163.1.1-7.1985 contributor: fullname: Schirch V. – ident: e_1_3_2_37_2 doi: 10.1074/jbc.R800048200 – ident: e_1_3_2_39_2 doi: 10.1038/nbt.1711 – ident: e_1_3_2_24_2 doi: 10.1021/bi2002289 – ident: e_1_3_2_22_2 doi: 10.1186/1752-0509-3-37 – ident: e_1_3_2_36_2 doi: 10.1093/nar/gkq1143 – ident: e_1_3_2_59_2 doi: 10.1038/msb.2010.47 – ident: e_1_3_2_30_2 doi: 10.1074/jbc.M111.274050 – ident: e_1_3_2_28_2 doi: 10.1038/nrg3033 – ident: e_1_3_2_4_2 doi: 10.1038/nrg2689 – volume: 18 start-page: 1529 year: 2008 ident: e_1_3_2_47_2 article-title: The diversity of lysine-acetylated proteins in Escherichia coli publication-title: J. Microbiol. Biotechnol. contributor: fullname: Yu B. J. – ident: e_1_3_2_45_2 doi: 10.1074/jbc.M309578200 – ident: e_1_3_2_33_2 doi: 10.1101/gr.3832305 – ident: e_1_3_2_21_2 doi: 10.1038/msb4100046 – ident: e_1_3_2_60_2 doi: 10.1371/journal.pcbi.1001050 – ident: e_1_3_2_6_2 doi: 10.1038/msb4100155 – ident: e_1_3_2_73_2 doi: 10.1074/jbc.271.44.27795 – ident: e_1_3_2_54_2 doi: 10.1006/jtbi.1993.1202 – ident: e_1_3_2_41_2 doi: 10.1016/j.jtbi.2007.12.008 – ident: e_1_3_2_18_2 doi: 10.1128/JB.00034-09 – ident: e_1_3_2_57_2 doi: 10.1128/JB.185.9.2692-2699.2003 – ident: e_1_3_2_11_2 doi: 10.1002/jez.b.21152 – ident: e_1_3_2_55_2 doi: 10.1002/(SICI)1097-0290(19971120)56:4<398::AID-BIT6>3.0.CO;2-J – ident: e_1_3_2_71_2 doi: 10.1046/j.0014-2956.2001.02606.x – ident: e_1_3_2_75_2 doi: 10.1016/S0168-6445(05)80006-3 – ident: e_1_3_2_43_2 doi: 10.1016/j.bpj.2010.12.3707 – volume: 9 start-page: 839 year: 2011 ident: e_1_3_2_17_2 article-title: Regulated proteolysis in Gram-negative bacteria—how and when? publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro2669 contributor: fullname: Gur E. – ident: e_1_3_2_27_2 doi: 10.1074/jbc.R111.254714 – ident: e_1_3_2_20_2 doi: 10.1016/j.copbio.2011.04.016 – volume: 118 start-page: 83 year: 1974 ident: e_1_3_2_62_2 article-title: Evolution of L-1, 2-propanediol catabolism in Escherichia coli by recruitment of enzymes for L-fucose and L-lactate metabolism publication-title: J. Bacteriol. doi: 10.1128/jb.118.1.83-88.1974 contributor: fullname: Cocks G. T. – ident: e_1_3_2_5_2 doi: 10.1021/bi101763c – ident: e_1_3_2_53_2 doi: 10.1002/bit.260350711 – ident: e_1_3_2_25_2 doi: 10.1146/annurev-genet-102209-163517 – ident: e_1_3_2_32_2 doi: 10.1038/nature02456 – volume: 261 start-page: 3363 year: 1986 ident: e_1_3_2_70_2 article-title: Properties of a serine hydroxymethyltransferase in which an active site histidine has been changed to an asparagine by site-directed mutagenesis publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)35791-5 contributor: fullname: Hopkins S. – volume: 10 start-page: 291 year: 2012 ident: e_1_3_2_8_2 article-title: Constraining the metabolic genotype-phenotype relationship using a phylogeny of in silico methods publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro2737 contributor: fullname: Lewis N. E. – ident: e_1_3_2_34_2 doi: 10.1038/nbt.1614 – ident: e_1_3_2_44_2 doi: 10.1016/S0006-3495(02)75150-3 – ident: e_1_3_2_2_2 doi: 10.1146/annurev.mi.30.100176.002205 – ident: e_1_3_2_67_2 doi: 10.1038/nature08112 – ident: e_1_3_2_76_2 doi: 10.1046/j.1365-2958.1998.00696.x – ident: e_1_3_2_52_2 doi: 10.1038/msb.2011.65 – ident: e_1_3_2_14_2 doi: 10.1038/ng1856 – ident: e_1_3_2_16_2 doi: 10.1038/msb4100162 – ident: e_1_3_2_38_2 doi: 10.1038/msb.2010.68 – ident: e_1_3_2_26_2 doi: 10.1371/journal.pcbi.0010068 – ident: e_1_3_2_31_2 doi: 10.1128/AEM.00373-10 – ident: e_1_3_2_42_2 doi: 10.1016/j.mib.2010.03.003 – ident: e_1_3_2_51_2 doi: 10.1093/nar/gkp896 – ident: e_1_3_2_12_2 doi: 10.1038/msb4100050 – ident: e_1_3_2_7_2 doi: 10.1038/ng1482 – ident: e_1_3_2_23_2 doi: 10.1038/msb.2011.52 – ident: e_1_3_2_78_2 doi: 10.1007/978-3-642-86659-3 – ident: e_1_3_2_15_2 doi: 10.1371/journal.pcbi.1000859 – ident: e_1_3_2_61_2 doi: 10.1038/nature01149 – ident: e_1_3_2_29_2 doi: 10.1016/j.copbio.2011.03.006 – ident: e_1_3_2_46_2 doi: 10.1073/pnas.0907176107 – ident: e_1_3_2_64_2 doi: 10.1126/science.1174671 – ident: e_1_3_2_58_2 doi: 10.1038/nbt1401 – volume: 170 start-page: 5330 year: 1988 ident: e_1_3_2_74_2 article-title: Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12 publication-title: J. Bacteriol. doi: 10.1128/jb.170.11.5330-5336.1988 contributor: fullname: Sawers G. – ident: e_1_3_2_35_2 doi: 10.1038/msb.2011.42 – ident: e_1_3_2_49_2 doi: 10.1074/mcp.M700311-MCP200 – ident: e_1_3_2_65_2 doi: 10.1371/journal.pcbi.1000938 – ident: e_1_3_2_13_2 doi: 10.1038/nature02636 – ident: e_1_3_2_77_2 doi: 10.1016/j.ymben.2003.09.002 – ident: e_1_3_2_19_2 doi: 10.1038/nchembio.495 – ident: e_1_3_2_72_2 doi: 10.1126/science.1188308 – ident: e_1_3_2_3_2 doi: 10.1146/annurev-biochem-030409-143718 – ident: e_1_3_2_63_2 doi: 10.1371/journal.pcbi.1000312 – ident: e_1_3_2_9_2 doi: 10.1038/nature02289 – ident: e_1_3_2_48_2 doi: 10.1074/mcp.M800187-MCP200 – ident: e_1_3_2_56_2 doi: 10.1073/pnas.97.10.5528 – volume: 6 start-page: 1290 year: 2011 ident: e_1_3_2_40_2 article-title: Quantitative prediction of cellular metabolism with constraint-based models: The COBRA Toolbox v2.0 publication-title: Nature Protocols doi: 10.1038/nprot.2011.308 contributor: fullname: Schellenberger J. – ident: e_1_3_2_66_2 doi: 10.1038/nchembio.186 – volume: 79 start-page: 471 year: 2010 ident: e_1_3_2_50_2 article-title: Enzyme promiscuity: A Mechanistic and evolutionary perspective publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev-biochem-030409-143718 contributor: fullname: Khersonsky O. – volume: 265 start-page: 14227 year: 1990 ident: e_1_3_2_68_2 article-title: Serine hydroxymethyltransferase catalyzes the hydrolysis of 5,10-methenyltetrahydrofolate to 5-formyltetrahydrofolate publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)77290-6 contributor: fullname: Stover P. – ident: e_1_3_2_79_2 doi: 10.1038/nrg2808 |
| SSID | ssj0009593 |
| Score | 2.548902 |
| Snippet | Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia... Good Enough Can Be Good Enough To begin to understand why some enzymes are promiscuous and have many substrates, whereas others are highly specific, and why... To begin to understand why some enzymes are promiscuous and have many substrates, whereas others are highly specific, and why some have high activity, whereas... Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia... |
| SourceID | pubmedcentral proquest crossref pubmed pascalfrancis jstor |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 1101 |
| SubjectTerms | Bacteria Bacteriology Biochemistry Biological and medical sciences Carbon Catalysis Chemistry Computational Biology Enzyme substrates Enzymes Enzymes - genetics Enzymes - metabolism Escherichia coli - enzymology Escherichia coli - genetics Evolution Evolution, Molecular Fundamental and applied biological sciences. Psychology Gene expression regulation Histograms Metabolic Networks and Pathways Metabolism Metabolism. Enzymes Microbiology Modeling Protein metabolism rev genes Selection, Genetic Substrate Specificity |
| Title | Network Context and Selection in the Evolution to Enzyme Specificity |
| URI | https://www.jstor.org/stable/23268972 https://www.ncbi.nlm.nih.gov/pubmed/22936779 https://www.proquest.com/docview/1037312483/abstract/ https://search.proquest.com/docview/1037656756 https://pubmed.ncbi.nlm.nih.gov/PMC3536066 |
| Volume | 337 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFLbKEBIXxAaDwJiMxGGoytTYiZ0cKyhUk-iFTeotimNH2yQStCYb25_DX8pz7CTO2KTBJapix0rzPr8f9uf3EPpAkjAnVGV-UCjmg__P_LjII58kgWIRF6FqqzV8W7HlSXi0jtaTyW-HtdTU4jC_ufNcyf9IFe6BXPUp2X-QbD8o3IDfIF-4goTh-iAZrwyHe9qmmPpluOLf28I2DoNxcWnfQbuZi_Lm-ocyVed17oh6tKvbTXTwOvudHEd-PSVxbogDHY_APuYsKqwMzJbVeWMtY8v5uTIJDVbtev10cTi0XNiF2KNqc9pk07nTpAz1XvlLpdw1Ck32iDvlbvWuTXtsrI5RtTNdJZLMqKuLqckAY0HHZqZCtVWu4KkEjqEObOHiv42AU7ZS6fQZLGbBYO-6Pf5bZrAnJ7ZhEWGpHSC1AzxCjwlPIh3gf10H9yZ2tumjnINZ3RuMPB9DftVM3GwDk7EwVVTuCnNus3Ud9-f4OXpm4xY8NyDcRhNV7qAnppLp9Q7athjY4AObyPzjC_TZ4hNbfGLAJ-7xic9KDGDCPT5xXWGDT-zg8yU6-bI4_rT0bdUOP49oUvtZomsf5QkTcZDPBM-JVDMmZCRg_gvKg4JKkiUxpfqbiSiWMszALoSSFYUQgu6irbIq1WuE4TGwf3kREpKHknMhA_ATJOOSqjyS0kMH3RdNf5rkLOk90vPQbvvF-34QSbA44cRD-yMRDB0YZQlYNA_tdTJJ7dzfpPp0LQXXOKYeet83g2bW221ZqarG9IFoiUfMQ6-MCIfBwctmnCce4iPh9h101vdxS3l22mZ_pxHViw5vHv7f36Knw6TcQ1v1RaPegStdi_0Wy38AYxPJiA |
| link.rule.ids | 230,315,786,790,891,27957,27958 |
| linkProvider | Geneva Foundation for Medical Education and Research |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Network+Context+and+Selection+in+the+Evolution+to+Enzyme+Specificity&rft.jtitle=Science+%28American+Association+for+the+Advancement+of+Science%29&rft.au=Nam%2C+Hojung&rft.au=Lewis%2C+Nathan+E.&rft.au=Lerman%2C+Joshua+A.&rft.au=Lee%2C+Dae-Hee&rft.date=2012-08-31&rft.issn=0036-8075&rft.eissn=1095-9203&rft.volume=337&rft.issue=6098&rft.spage=1101&rft.epage=1104&rft_id=info:doi/10.1126%2Fscience.1216861&rft.externalDBID=n%2Fa&rft.externalDocID=10_1126_science_1216861 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0036-8075&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0036-8075&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0036-8075&client=summon |