Cloning and Characterization of a Coronatine-Regulated Tyrosine Aminotransferase from Arabidopsis

• Published in: Plant physiology (Bethesda) Vol. 126; no. 4; pp. 1678 - 1687
• Main Authors: Anna Lopukhina, Marcus Dettenberg, Weiler, Elmar W., Holländer-Czytko, Heike
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.08.2001; American Society of Plant Physiologists
• Subjects: 12-oxo-phytodienoic acid; Amino Acid Sequence; Amino Acids - physiology; Animal and plant ecology; Animal, plant and microbial ecology; Animals; Antiserum; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - enzymology; Arabidopsis - genetics; Autoecology; Biological and medical sciences; Cell Biology and Signal Transduction; Cloning; Cloning, Molecular; Complementary DNA; coronatine; DNA, Complementary; DNA, Plant; Enzymatic activity; Enzymes; Fundamental and applied biological sciences. Psychology; Gels; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Generalities. Disease free stocks; Genes; Herbivores; Humans; Indenes; jasmonic acid; Messenger RNA; Molecular Sequence Data; Physiological responses; Physiology; Phytopathology. Animal pests. Plant and forest protection; Phytotoxins; Plant Diseases; Plants; Plants and fungi; Polymerase chain reaction; Proteins; RNA; RNA, Messenger; RNA, Plant; Stearic Acids - metabolism; Stearic Acids - pharmacology; TAT gene; tyrosine aminotransferase; Tyrosine Transaminase - genetics; Tyrosine Transaminase - isolation & purification; Tyrosine Transaminase - metabolism; Terpenoid; Phytotoxin; Plant pathogen; Enzyme; Induction; Transferases; Injury; Tyrosine transaminase; Host agent relation; Gene expression; Biological activity; Arabidopsis thaliana; Characterization; Regulation(control); Pest; Cruciferae; Analog; Dicotyledones; Transaminases; Angiospermae; Defense mechanism; Spermatophyta; Experimental plant; Molecular cloning
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.126.4.1678

In plants, the phytotoxin coronatine, which is an analog of the octadecanoids 12-oxo-phytodienoic acid and/or jasmonic acid, gives rise to a number of physiological responses similar to those of octadecanoids. To further elucidate the physiological role of these compounds, the differential RNA display technique was used to isolate a number of novel octadecanoid-inducible genes expressed in coronatine-treated Arabidopsis. Among these, a cDNA clone was identified that was similar to known tyrosine aminotransferases (TATs). The function was verified with the expressed recombinant protein. In Arabidopsis, the protein is present as a multimer of 98 kD, with a monomer of an apparent molecular mass of 47 kD. TAT mRNA could be induced within 2 h by various octadecanoids and by wounding of the plants. Accumulation of the TAT protein and a 5- to 7-fold increase in its enzymatic activity was observed 7 to 9 h after application of octadecanoids, coronatine, or wounding. The potential role of TAT in the defense response to herbivores and pathogens is discussed.