Assessment of genotoxicity and cytotoxicity of standardized aqueous extract from leaves of Erythroxylum cuneatum in human HepG2 and WRL68 cells line

• Published in: Asian Pacific journal of tropical medicine Vol. 6; no. 10; pp. 811 - 816
• Main Authors: Wesam, RK, Ghanya, AN, Mizaton, HH, ILham, M, Aishah, A
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.10.2013
• Subjects: Cell Line, Tumor; Cell Proliferation - drug effects; Comet Assay; cuneatu; Cytotoxicity; Cytotoxins - toxicity; damage; DNA; DNA damage; DNA Damage - drug effects; Erythroxylaceae - chemistry; Erythroxylum; Erythroxylum cuneatu; Genotoxicity; Hep G2 Cells; HepG2; Humans; Mutagens - toxicity; Plant Extracts - toxicity; Plant Leaves - chemistry; WRL68; HepG2; Cytotoxicity; WRL68; DNA damage; Erythroxylum cuneatu; Genotoxicity
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/s1995-7645(13)60143-1

Objective:To investigate the cytotoxicity and the genotoxicity of standardized aqueous of dry leaves of Erythroxylum cuneatum（E.cuneatum）in human HepG2 and WRL68 cells.Methods:The cytoloxicity of E.cuneatum extract was evaluated by both MTS and LDH assays.Genotoxicity study on E.cuneatum extract was assessed by the single cell gel electrophoresis（comet assay）.The protective effect of E.cuneatum against menadione-induced cytotoxicity was also investigated.Results:Results from this study showed that E.cuneatum extract exhibited cytotoxic activities towards the cells with IC50value of（125±12）and（125±14）μg/mL for HepG2and WRL68 cells respectively,after 72 h incubation period as determined by MTS assay.LDH leakage was detected at（251±19）and（199.5±12.0）μg/mL for HepG2 and WRL68 respectively.Genotoxicity study results showed that treatment with E.cuneatum up to 1 mg/mL did not cause obvious DNA damage in WRL68 and HepG2 cells.Addition of E.cunaetum did not show significant protection towards menadione in WRL68 and HepG2 Cells.Conclusions:E.cuneatum standardized aqueous extract might be developed in onler to establish new pharmacological possibilities for its application.