miR-1224 inhibits cell proliferation in acute liver failure by targeting the antiapoptotic gene Nfib

• Published in: Journal of hepatology Vol. 67; no. 5; pp. 966 - 978
• Main Authors: Roy, Sanchari, Bantel, Heike, Wandrer, Franziska, Theres Schneider, Anne, Gautheron, Jeremie, Vucur, Mihael, Tacke, Frank, Trautwein, Christian, Luedde, Tom, Roderburg, Christoph
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017; Elsevier Science Ltd; Elsevier
• Subjects: Acute liver failure; Adult; Animal models; Animals; Apoptosis; Biomarker; Biomarkers; Biomarkers - metabolism; Bromodeoxyuridine - metabolism; Cell death; Cell Death - physiology; Cell growth; Cell proliferation; Cell Proliferation - physiology; Clinical outcomes; Clinical trials; DNA Nucleotidylexotransferase - metabolism; Female; Gastroenterology and Hepatology; Hepatocytes; Hepatocytes - metabolism; Humans; Hydrogen peroxide; Ischemia; Labeling; Life Sciences; Liver diseases; Liver failure; Liver Failure, Acute - etiology; Liver Failure, Acute - genetics; Liver Failure, Acute - metabolism; Male; Mice; MicroRNAs; MicroRNAs - metabolism; Middle Aged; miR-1224; miRNA; Nfib; Oxidative stress; Proliferation; Reperfusion; Reperfusion Injury - complications; Reperfusion Injury - metabolism; Reproducibility of Results; RNA-mediated interference; Serum levels; Stains & staining; Up-Regulation; APAP; small interfering RNA; MTT; I/R; TNF; arbritary units; AUC; UTR; TUNEL; miR-1224; 5-bromo-2'-deoxyuridine; qRT-PCR; terminal deoxynucleotide transferase nick end labeling; untranslated region; NSR; biomarker; cl. CASP-3; H 2O 2; acetaminophen; AU; ROS; hypoxia/regeneration; GAPDH; Area under curve; Hydrogen peroxide; spontaneous recovery; Carbon tetrachloride; cleaved Caspase-3; mRNA; H/R; messenger RNA; Nfib; reactive oxygen species; miRNA; non-spontaneous recovery; glyceraldehyde-3-phosphate dehydrogenase; quantitative real-time polymerase chain reaction; SR; methyl thiazol tetrazolium; Acute liver failure; cell death; proliferation; siRNA; Ischemia-reperfusion; CCl 4; tumor necrosis factor; microRNA; ALF; BrdU; oxidative stress; Oxidative stress; Biomarker; Cell death; Proliferation; APAP, acetaminophen; miR-1224 Acute liver failure Cell death Proliferation Biomarker Nfib Oxidative stress; AU, arbritary units; UTR, untranslated region; H 2 O 2 , Hydrogen peroxide; CCl 4 , Carbon tetrachloride; qRT-PCR, quantitative real-time polymerase chain reaction; SR, spontaneous recovery; H/R, hypoxia/regeneration; NSR, non- spontaneous recovery; ROS, reactive oxygen species; MTT, methyl thiazol tetrazolium; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR-1224, acute liver failure, cell death, proliferation, biomarker, Nfib, oxidative stress ALF, Acute liver failure I/R, Ischemia-reperfusion miRNA, microRNA APAP, acetaminophen H 2 O 2 , Hydrogen peroxide CCl 4 , Carbon tetrachloride BrdU, 5-bromo-2'-deoxyuridine AUC, Area under curve SR, spontaneous recovery NSR, non- spontaneous recovery siRNA, small interfering RNA mRNA, messenger RNA qRT-PCR, quantitative real-time polymerase chain reaction AU, arbritary units H/R, hypoxia/regeneration TNF, tumor necrosis factor MTT, methyl thiazol tetrazolium cl. CASP-3, cleaved Caspase-3 GAPDH, glyceraldehyde-3-phosphate dehydrogenase UTR, untranslated region TUNEL, terminal deoxynucleotide transferase nick end labeling ROS, reactive oxygen species; miR-1224, acute liver failure, cell death, proliferation, biomarker, Nfib, oxidative stress ALF, Acute liver failure; mRNA, messenger RNA; TNF, tumor necrosis factor; miRNA, microRNA; TUNEL, terminal deoxynucleotide transferase nick end labeling; siRNA, small interfering RNA; AUC, Area under curve; BrdU, 5-bromo-2'-deoxyuridine; cl. CASP-3, cleaved Caspase-3; I/R, Ischemia-reperfusion
• ISSN: 0168-8278; 1600-0641
• DOI: 10.1016/j.jhep.2017.06.007

Graphical abstract