Replication of Enterotropic and Polytropic Murine Coronaviruses in Cultured Cell Lines of Mouse Origin

• Published in: Experimental Animals Vol. 49; no. 4; pp. 251 - 257
• Main Authors: KYUWA, Shigeru, OHSAWA, Kazutaka, SATO, Hiroshi, URANO, Toru
• Format: Journal Article
• Language: English
• Published: Japan Japanese Association for Laboratory Animal Science 2000
• Subjects: Animals; B16 cells; Cell Line; Coronavirus - physiology; IC-21 cells; Kinetics; Melanoma, Experimental; Mice; murine coronavirus; Murine hepatitis virus - physiology; Rectal Neoplasms; replication; Stem Cells; TCID50; Tumor Cells, Cultured; Viral Plaque Assay; Virus Replication
• ISSN: 1341-1357; 1881-7122
• DOI: 10.1538/expanim.49.251

To understand the virus-cell interactions that occur during murine coronavirus infection, six murine cell lines (A3-1M, B16, CMT-93, DBT, IC-21 and J774A.1) were inoculated with eight murine coronaviruses, including prototype strains of both polytropic and enterotropic biotypes, and new isolates. All virus strains produced a cytopathic effect (CPE) with cell-to-cell fusion in B16, DBT, IC-21 and J774A.1 cells. The CPE was induced most rapidly in IC-21 cells and was visible microscopically in all cell lines tested. In contrast, the coronaviruses produced little CPE in A3-1M and CMT-93 cells. Although most virus-infected cells, except KQ3E-infected A3-1M, CMT-93 and J774A.1 cells, produced progeny viruses in the supernatants when assayed by plaque formation on DBT cells, the kinetics of viral replication were dependent on both the cell line and virus strain; replication of prototype strains was higher than that of new isolates. There was no significant difference in replication of enterotropic and polytropic strains. B16 cells supported the highest level of viral replication. To determine the sensitivity of the cell lines to murine coronaviruses, the 50% tissue culture infectious dose of the coronaviruses was determined with B16, DBT, IC-21 and J774A.1 cells, and compared to that with DBT cells. The results indicate that IC-21 cells were the most sensitive to murine coronaviruses. These data suggest that B16 and IC-21 cells are suitable for large-scale preparation and isolation of murine coronaviruses, respectively.