Biodegradation of phenol by free and immobilized Acinetobacter sp. strain PD12

• Published in: Journal of environmental sciences (China) Vol. 19; no. 2; pp. 222 - 225
• Main Authors: WANG, Ying, TIAN, Ye, HAN, Bin, ZHAO, Hua-bing, BI, Jian-nan, CAI, Bao-li
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2007
• Subjects: Acinetobacter; Acinetobacter - drug effects; Acinetobacter - genetics; Acinetobacter - isolation & purification; Acinetobacter - metabolism; Acinetobacter sp. PD12; biodegradation; Biodegradation, Environmental; Catechol 2,3-Dioxygenase - metabolism; DNA, Bacterial - genetics; Hydrogen-Ion Concentration; immobilized bacterium; PD12; phenol; Phenol - metabolism; Polyvinyl Alcohol - pharmacology; RNA, Ribosomal, 16S - genetics; Temperature; Water Pollutants, Chemical - metabolism; 环境保护; 生物降解; 石碳酸; China, People's Rep; China, People's Rep., Tianjin; Acinetobacter sp. PD12; phenol; biodegradation; immobilized bacterium
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/S1001-0742(07)60036-9

A new phenol-degrading bacterium with high biodegradation activity and high tolerance of phenol, strain PD 12, was isolated from the activated sludge of Tianjin Jizhuangzi Wastewater Treatment Facility in China. This strain was capable of removing 500 mg phenol/L in liquid minimal medium by 99.6% within 9 h and metabolizing phenol at concentrations up to 1100 mg/L. DNA sequencing and homologous analysis of 16S rRNA gene identified PD12 to be an Acinetobacter sp. Polyvinyl alcohol （PVA） was used as a gel matrix to immobilize Acinetobacter sp. strain PDI2 by repeated freezing and thawing. The factors affecting phenol degradation of immobilized cells were investigated, and the results showed that the immobilized cells could tolerate a high phenol level and protected the bacteria against changes in temperature and pH. Storage stability and reusability tests revealed that the phenol degradation functions of immobilized cells were stable after reuse for 50 times or storing at 4℃ for 50 d. These results indicate that immobilized Acinetobacter sp. strain PD 12 possesses a good application potential in the treatment of phenol-containing wastewater.