Isolating a cytoprotective compound from Ganoderma tsugae: Effects on induction of Nrf-2-related genes in endothelial cells

• Published in: Bioscience, biotechnology, and biochemistry Vol. 73; no. 8; pp. 1757 - 1763
• Main Authors: Wei, Y.S.(National Chiayi Univ. (Taiwan)), Wung, B.S, Lin, Y.C, Hsieh, C.W
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.08.2009; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: Animals; ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; Biological and medical sciences; Biological Assay; Cardiotonic Agents - isolation & purification; Cardiotonic Agents - pharmacology; CARDIOVASCULAR DISEASES; Cattle; Cytoprotection; endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - metabolism; ENFERMEDADES CARDIOVASCULARES; ENZIMAS; ENZYME; ENZYMES; EXPRESION GENICA; EXPRESSION DES GENES; FACTEUR DE TRANSCRIPTION; FACTORES DE TRANSCRIPCION; FERMENTACION; FERMENTATION; Fundamental and applied biological sciences. Psychology; GANODERMA; Ganoderma - chemistry; Ganoderma tsugae; GENE EXPRESSION; Gene Expression Regulation, Enzymologic - drug effects; Genes, Reporter; Glycoproteins - isolation & purification; Glycoproteins - pharmacology; heme oxygenase-1; Heme Oxygenase-1 - genetics; Humans; Luciferases - genetics; MALADIE CARDIOVASCULAIRE; NF-E2-Related Factor 2 - genetics; NF-E2-related factor-2; Oxidative Stress - drug effects; peptidoglycan; Peptidoglycan - isolation & purification; Peptidoglycan - pharmacology; Protein Transport - drug effects; PROTEOGLICANOS; PROTEOGLYCANE; PROTEOGLYCANS; Thioredoxins - genetics; TRANSCRIPTION FACTORS; Transcriptional Activation - drug effects; Endothelial cell; endothelial cells; Enzyme; Induction; Ganoderma tsugae; heme oxygenase-1; NF-E2-related factor-2; Basidiomycota; Fungi; Oxygenase; Peptidoglycan; Gene; Heme; Oxidoreductases; Ganoderma
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.90098

Ganoderma tsugae is a medicinal fungus with several biological activities. It has long been used as a folk remedy for the promotion of health and longevity in China and other oriental countries. Here, a bioactive fraction of G. tsugae was progressively purified to be enriched in the activity of cytoprotective enzymes. The highest bioactivity was detected in the 20% EtOH-precipitated fraction, which was prepared from submerged fermentation filtrate of G. tsugae. Following further purification by gel filtration chromatography and acetone extraction, the most bioactive fraction, F5-2, was identified as a peptidoglycan-like compound. Extracts of G. tsugae (F5-2) induced heme oxygenase-1 (HO-1) and thioredoxin reductase-1 (TrxR1) expression in endothelial cells by increasing NF-E2-related factor-2 (Nrf2) nuclear translocation. Pretreatment with F5-2 increased intracellular glutathione (GSH) and protected against H 2 O 2 , suggesting that induction of these antioxidant enzymes is important in protection against oxidative stress. Hence the bioactive peptidoglycan-like compound from G. tsugae might protect endothelial cells.