Surface Properties of Parabacteroides distasonis and Impacts of Stress-Induced Molecules on Its Surface Adhesion and Biofilm Formation Capacities

• Published in: Microorganisms (Basel) Vol. 9; no. 8; p. 1602
• Main Authors: Chamarande, Jordan, Cunat, Lisiane, Caillet, Céline, Mathieu, Laurence, Duval, Jérôme F L, Lozniewski, Alain, Frippiat, Jean-Pol, Alauzet, Corentine, Cailliez-Grimal, Catherine
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.07.2021; MDPI
• Subjects: Adhesion; adhesion and biofilm capacities; Bacteria; Biofilms; Cell division; Dilution; Electrokinetics; Electrolytes; Enzymes; Experiments; gut microbiota; Homeostasis; Hormones; Intestinal microflora; Laboratories; Life Sciences; Maintenance; Metabolism; Microbiology and Parasitology; Microbiota; Parabacteroides distasonis; Physiology; stress; Substrates; Surface properties; France; Parabacteroides distasonis; stress; adhesion and biofilm capacities; gut microbiota
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms9081602

The gut microbiota is a complex and dynamic ecosystem whose balance and homeostasis are essential to the host's well-being and whose composition can be critically affected by various factors, including host stress. causes well-known beneficial roles for its host, but is negatively impacted by stress. However, the mechanisms explaining its maintenance in the gut have not yet been explored, in particular its capacities to adhere onto (bio)surfaces, form biofilms and the way its physicochemical surface properties are affected by stressing conditions. In this paper, we reported adhesion and biofilm formation capacities of 14 unrelated strains of using a steam-based washing procedure, and the electrokinetic features of its surface. Results evidenced an important inter-strain variability for all experiments including the response to stress hormones. In fact, stress-induced molecules significantly impact adhesion and biofilm formation capacities in 35% and 23% of assays, respectively. This study not only provides basic data on the adhesion and biofilm formation capacities of to abiotic substrates but also paves the way for further research on how stress-molecules could be implicated in maintenance within the gut microbiota, which is a prerequisite for designing efficient solutions to optimize its survival within gut environment.