Increased Tissue Neutral Endopeptidase 24.11 Activity in Spontaneously Hypertensive Hamsters
The purpose of this study was to determine whether tissue neutral endopeptidase (NEP) 24.11 activity, a membrane-bound metalloenzyme widely distributed in the peripheral circulation that cleaves and inactivates vasodilator peptides, is increased in spontaneously hypertensive hamsters relative to gen...
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Published in | American journal of hypertension Vol. 11; no. 5; pp. 585 - 590 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Elsevier Inc
01.05.1998
Oxford University Press Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | The purpose of this study was to determine whether tissue neutral endopeptidase (NEP) 24.11 activity, a membrane-bound metalloenzyme widely distributed in the peripheral circulation that cleaves and inactivates vasodilator peptides, is increased in spontaneously hypertensive hamsters relative to genetically/age-matched normotensive hamsters. Mean arterial pressure and heart rate were 163 ± 11 mm Hg and 312 ± 7 beats/min in spontaneously hypertensive hamsters and 99 ± 3 mm Hg and 302 ± 10 beats/min in normotensive hamsters, respectively (mean ± SEM). NEP 24.11 activity is significantly increased in the kidney, cheek pouch, and spinotrapezius muscle, and significantly decreased in the heart and aorta of spontaneously hypertensive hamsters relative to controls (
P < .05). Lung and brain NEP 24.11 activity is similar in both groups. Renal NEP 24.11 activity increases and to a similar extent in spontaneously hypertensive and normotensive hamsters as chloride anion concentration in the assay buffer is increased. Substituting citrate for chloride anion significantly attenuates renal NEP 24.11 activity. Taken together, these data indicate that NEP 24.11 activity in spontaneously hypertensive hamsters is increased in two organs that contribute appreciably to peripheral vascular resistance, skeletal muscle, and kidney. We suggest that the spontaneously hypertensive hamster is a suitable model to study the role of skeletal muscle and renal NEP 24.11 in regulating vasomotor tone in essential hypertension. |
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Bibliography: | This study was supported, in part, by grants from the National Institutes of Health (DE10347 and GM46459) and American Heart Association of Metropolitan Chicago. Dr. Rubinstein is a recipient of a Research Career Development Award from the National Institutes of Health (DE00386) and a University of Illinois Scholar Award. istex:018143F86EF6747250D7F71F28AEFA6D9C086765 ark:/67375/HXZ-3J4NT5WG-T href:11_5_585.pdf ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0895-7061 1879-1905 1941-7225 |
DOI: | 10.1016/S0895-7061(98)00028-4 |