Identification and distribution of nitric oxide synthase in the brain of adult zebrafish

• Published in: Neuroscience letters Vol. 292; no. 2; pp. 119 - 122
• Main Authors: Holmqvist, Bo, Ellingsen, Berit, Alm, Per, Forsell, Johan, Øyan, Anne-Margrete, Goksøyr, Anders, Fjose, Anders, Seo, Hee-Chan
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 06.10.2000; Elsevier
• Subjects: Age Factors; Anatomy; Animals; Biological and medical sciences; Central nervous system; Danio rerio; Digoxigenin; Digoxigenin-in situ hybridization; Freshwater; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Immunocytochemistry; In Situ Hybridization; Molecular Sequence Data; NADPH Dehydrogenase - analysis; Nicotinamide adenine dinucleotide phosphate diaphorase histochemistry; Nitric Oxide Synthase - analysis; Nitric Oxide Synthase - genetics; Nitric Oxide Synthase Type I; Nitric oxide synthase type-1; Regeneration; Reverse Transcriptase Polymerase Chain Reaction; Reverse transcription-polymerase chain reaction; RNA, Messenger - analysis; Sequence Homology, Amino Acid; Telencephalon - enzymology; Teleostei; Vertebrates: nervous system and sense organs; Zebrafish; Nitric oxide synthase type-1; Regeneration; Digoxigenin-in situ hybridization; Nicotinamide adenine dinucleotide phosphate diaphorase histochemistry; Immunocytochemistry; Central nervous system; Teleostei; Reverse transcription-polymerase chain reaction; Vertebrata; Brachydanio rerio; Enzyme; Pisces; Nitric oxide; Distribution; Oxidoreductases; Nitric-oxide synthase; Brain (vertebrata)
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(00)01460-9

Nitric oxide (NO) is proposed to be involved in developmental and plastic processes. We investigated the presence and distribution of nitric oxide synthase (NOS) in the zebrafish ( Danio rerio) using molecular and histochemical techniques. A partial gene sequence corresponding to the neuronal NOS isoform (nNOS) was identified, and in situ hybridization revealed cellular nNOS mRNA expression throughout the brain of adult zebrafish, distributed in distinct central nuclei and in proliferation zones. NOS immunoreactivity and nicotinamide adenine dinucleotide phosphate diaphorase activity partly coincided with the nNOS mRNA expression, however was present also in additional neuronal and non-neuronal cell types. The results indicate the occurrence of different NOS isoforms in the adult brain, of which nNOS may participate in neurotransmission, and in mechanisms related to the continuous growth and neuronal plasticity of the teleost brain.