Inducible bacteriophages from ruminal bacteria

The incidence of temperate bacteriophage in a wide range of ruminal bacteria was investigated by means of induction with mitomycin C. Supernatant liquid from treated cultures was examined for phagelike particles by using transmission electron microscopy. Of 38 ruminal bacterial studied, nine organis...

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Bibliographic Details
Published inApplied and Environmental Microbiology Vol. 55; no. 6; pp. 1630 - 1634
Main Authors Klieve, A.V. (University of New England, Armidale, New South Wales, Australia), Hudman, J.F, Bauchop, T
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.06.1989
Subjects
Animal, plant and microbial ecology
Animals
ANTIBIOTICOS
ANTIBIOTIQUE
BACTERIA
Bacteria - isolation & purification
BACTERIE
BACTERIOFAGOS
BACTERIOPHAGE
Bacteriophages - drug effects
Bacteriophages - isolation & purification
Bacteriophages - ultrastructure
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbial ecology
Microscopy, Electron
Mitomycin
Mitomycins - pharmacology
Normal microflora of man and animals. Rumen
RUMEN
Rumen - microbiology
Sheep - microbiology
Virus Activation - drug effects
Virus
Transmission electron microscopy
Butyrivibrio fibrisolvens
Induction
Production
Bacteria
Chromosome
Genetics
Rumen
Genome
Phage
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Summary:The incidence of temperate bacteriophage in a wide range of ruminal bacteria was investigated by means of induction with mitomycin C. Supernatant liquid from treated cultures was examined for phagelike particles by using transmission electron microscopy. Of 38 ruminal bacterial studied, nine organisms (23.7%) representing five genera (Eubacteria, Bacteroides, Butyrivibrio, Ruminococcus, and Streptococcus) produced phagelike particles. Filamentous particles from Butyrivibrio fibrisolvens are the first of this morphological type reported from ruminal bacteria. All of the other particles obtained possessed polyhedral heads and long, noncontractile tails (group B-type phage). The limited range of morphological types produced by mitomycin C induction cannot yet account for the much wider range of types found in ruminal contents by direct examination. The presence of viral genetic material in a significant percentage of the bacteria tested, as well as in a range of different genera, indicates that viral genetic material may be a normal constituent of the genome of appreciable numbers of ruminal bacteria
Bibliography:L51
9011695
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.55.6.1630-1634.1989