Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection

• Published in: Journal of clinical virology Vol. 69; pp. 190 - 196
• Main Authors: Fukutani, Kiyoshi F, Nascimento-Carvalho, Cristiana M, Van der Gucht, Winke, Wollants, Elke, Khouri, Ricardo, Dierckx, Tim, Van Ranst, Marc, Houspie, Lieselot, Bouzas, Maiara L, Oliveira, Juliana R, Barral, Aldina, Van Weyenbergh, Johan, de Oliveira, Camila I
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2015
• Subjects: Allergy and Immunology; ARI; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacterial Infections - diagnosis; Chlamydophila pneumoniae; Diagnostics; Gene Expression Profiling - methods; Haemophilus influenzae; Human metapneumovirus; Humans; Infant; Infectious Disease; Influenza virus; Moraxella catarrhalis; Multiplex Polymerase Chain Reaction - methods; Mycoplasma pneumoniae; Nasopharynx - microbiology; nCounter; Parainfluenza virus; Phylogeny; Respiratory Tract Infections - diagnosis; Respiratory Tract Infections - microbiology; Rhinovirus; RNA, Bacterial - analysis; RNA, Viral - analysis; RSV; Sensitivity and Specificity; Staphylococcus aureus; Streptococcus pneumoniae; Virus Diseases - diagnosis; Viruses - classification; Viruses - genetics; Viruses - isolation & purification; ARI; Diagnostics; RSV; nCounter
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2015.06.005

Highlights • nCounter enables detection of pathogen transcripts in NPA with low RNA input. • nCounter detects, in a single reaction, the presence of multiple pathogens in NPA. • nCounter displayed a good agreement with Real-Time PCR for RSV.