Dual-acting antibacterial porous chitosan film embedded with a photosensitizer

• Published in: Science and technology of advanced materials Vol. 21; no. 1; pp. 562 - 572
• Main Authors: Liu, Zi-Jun, Lin, Shu-Ching, Lee, Pei-Yuan, Lin, Ying-Ting, Lai, Zi-Lun, Chang, Cheng-Chung, Wang, Gou-Jen
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 31.01.2020; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: 102 Porous / Nanoporous / Nanostructured materials; 211 Scaffold / Tissue engineering/Drug delivery; Absorption; Antiinfectives and antibacterials; bactericidal and bacteriostatic effects; Bio-Inspired and Biomedical Materials; Cell growth; Chitosan; Compounds; Dual-acting antibacterial film; Exudation; Fibroblasts; Growth factors; Light irradiation; Moisture absorption; Photodynamic therapy; Photosensitivity; Porosity; porous chitosan/small molecular compound; Toxicity; Wound healing
• ISSN: 1468-6996; 1878-5514
• DOI: 10.1080/14686996.2020.1795431

This study proposes to develop a dual-acting antibacterial film of porous chitosan (Cs) embedded with small molecular compound, which possesses photosensitive characteristics with bactericidal efficacy, to promote the accelerated recovery of infectious wounds. The Cs/small molecular compound (Cs-cpd.2) dressing was prepared using the freeze-drying method. Characterization of the synthesized Cs-cpd.2 indicated that it has high porosity and moisture absorption effect, hence enhancing the absorption of wound exudate. Experimental results showed that Cs-cpd.2 dressing has good bactericidal and bacteriostatic effects on Staphylococcus aureus under visible-light irradiation and has antibacterial effect in the dark. It was also found that the small molecular compound does not have cytotoxicity at a dose of 0-5 μM. Furthermore, Cs-cpd.2 that contained small molecular compound with a concentration of 0.3-1 μM has positive effect on both the cell viability rate and cell proliferation rate of human fibroblast CG1639. Cs-cpd.2 can significantly promote cell proliferation when the small molecular compound and the basic fibroblast growth factor bFGF were added together. Therefore, the proposed Cs-cpd.2 dressing is feasible for photodynamic therapy (PDT) and clinical wound dressing applications.