Abscissic acid specific expression of RAB18 involves activation of anion channels in Arabidopsis thaliana suspension cells

• Published in: FEBS letters Vol. 474; no. 1; pp. 43 - 47
• Main Authors: Ghelis, Thanos, Dellis, Olivier, Jeannette, Emmanuelle, Bardat, Françoise, Cornel, Daniel, Miginiac, Emile, Rona, Jean-Pierre, Sotta, Bruno
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 26.05.2000
• Subjects: Abscisic Acid - pharmacology; Abscissic acid perception; Anion current; Anions; Arabidopsis - physiology; Arabidopsis Proteins; Arabidopsis thaliana suspension cell; Barium Compounds - pharmacology; Chlorides - pharmacology; Electric Conductivity; Gene Expression - drug effects; Ion Channels - antagonists & inhibitors; Ion Channels - physiology; K + inward rectifying current; Patch-Clamp Techniques; Plant Proteins - genetics; Potassium Channel Blockers; Potassium Channels - physiology; rab GTP-Binding Proteins; RAB18; Signal Transduction; Space life sciences; Tetraethylammonium - pharmacology; Zinc Sulfate - pharmacology; Abscissic acid perception; K + inward rectifying current; RAB18; Arabidopsis thaliana suspension cell; Anion current
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(00)01574-X

The abscissic acid (ABA) transduction cascade following the plasmalemma perception was analyzed in intact Arabidopsis thaliana suspension cells. In response to impermeant ABA, anion currents were activated and K + inward rectifying currents were inhibited. Anion current activation was required for the ABA specific expression of RAB18. By contrast, specific inhibition of K + channels by tetraethylammonium or Ba 2+ did not affect RAB18 expression. Thus, outer plasmalemma ABA perception triggered two separated signaling pathways.