Phosphatase-Triggered Fusogenic Liposomes for Cytoplasmic Delivery of Cell-Impermeable Compounds

License to fuse! A phosphorylated fusion peptide can mediate membrane fusion when the phosphates (green triangles, see scheme) are removed by phosphatases (blue spheres), delivering the contents of the liposome into the cytosol. This phosphatase‐triggered approach may be useful to create target‐spec...

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Published inAngewandte Chemie (International ed.) Vol. 51; no. 36; pp. 9047 - 9051
Main Authors Motion, J. P. Michael, Nguyen, Juliane, Szoka, Francis C.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 03.09.2012
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
Animals
Cell Line, Tumor
Cell Survival - drug effects
Cytoplasm - metabolism
enzymes
Fuses
fusion peptides
HIV Envelope Protein gp41 - chemistry
Humans
Licenses
Lipids
Liposomes
Liposomes - chemistry
Liposomes - toxicity
membrane fusion
Membranes
Mice
Nanostructure
Peptides
Peptides - metabolism
Phosphates
Phosphoric Monoester Hydrolases - chemistry
Phosphoric Monoester Hydrolases - metabolism
Transfection
Triangles
triggered release
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Summary:License to fuse! A phosphorylated fusion peptide can mediate membrane fusion when the phosphates (green triangles, see scheme) are removed by phosphatases (blue spheres), delivering the contents of the liposome into the cytosol. This phosphatase‐triggered approach may be useful to create target‐specific lipid nanocarriers.
Bibliography:ark:/67375/WNG-VVRVV8W1-V
Deutsche Forschungsgemeinschaft
DFG
istex:B6AA62A0C4C631F8F4DAC20A62C6A7A9B20ADD9F
This work was supported by the National Institute of Health (R01GM003008). J.P.M.M. is a recipient of the NIGMS predoctoral fellowship, supported by the NIGMS-IMSD grant (R25-GM56847). J.N. is supported by the Deutsche Forschungsgemeinschaft Fellowship (DFG). We thank Dr. Justin Chan and Dr. David Pham for their assistance with peptide synthesis and Sebastian Peck and Jessica Wong from the Biological Imaging Development Center at UCSF for their assistance in confocal microscopy.
National Institute of Health - No. R01GM003008
ArticleID:ANIE201204198
NIGMS-IMSD - No. R25-GM56847
This work was supported by the National Institute of Health (R01GM003008). J.P.M.M. is a recipient of the NIGMS predoctoral fellowship, supported by the NIGMS‐IMSD grant (R25‐GM56847). J.N. is supported by the Deutsche Forschungsgemeinschaft Fellowship (DFG). We thank Dr. Justin Chan and Dr. David Pham for their assistance with peptide synthesis and Sebastian Peck and Jessica Wong from the Biological Imaging Development Center at UCSF for their assistance in confocal microscopy.
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SourceType-Scholarly Journals-1
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201204198