Serial Expression of the Truncated Fragments of the Nucleocapsid Protein of CCHFV and Identification of the Epitope Region

• Published in: Virologica Sinica Vol. 25; no. 1; pp. 45 - 51
• Main Authors: Wei, Peng-fei, Luo, Yan-jun, Li, Tian-xian, Wang, Hua-lin, Hu, Zhi-hong, Zhang, Fu-chun, Zhang, Yu-jiang, Deng, Fei, Sun, Su-rong
• Format: Journal Article
• Language: English; Chinese
• Published: Heidelberg SP Wuhan Institute of Virology, CAS 01.02.2010; KeAi Publishing Communications Ltd; Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China%State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China%The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi 830002,China
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Biochemistry; Biomedical and Life Sciences; Biomedicine; Blotting, Western; Crimean hemorrhagic fever; Crimean-Congo hemorrhagic fever virus; Epidemiology; Epitope Mapping - methods; Epitopes; Epitopes - genetics; Epitopes - immunology; Escherichia coli - genetics; Expression vectors; Gene Expression; Hemorrhage; Hemorrhagic Fever Virus, Crimean-Congo - genetics; Hemorrhagic Fever Virus, Crimean-Congo - immunology; Medical Microbiology; Microbial Genetics and Genomics; Microbiology; Mutant Proteins - genetics; Mutant Proteins - immunology; NP protein; Nucleocapsid Proteins - genetics; Nucleocapsid Proteins - immunology; Nucleocapsids; Oncology; Proteins; Rabbits; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Sequence Deletion; Virology; 单克隆抗体; 抗原表位; 核衣壳; 衣壳蛋白; Epitope; Nucleocapsid protein (NP); Expression; Crimean-congo hemorrhagic fever virus (CCHFV); Crimean-congo hemorrhagic fever virus(CCHFV); Nucleocapsid protein(NP)
• ISSN: 1674-0769; 1995-820X
• DOI: 10.1007/s12250-010-3067-7

The Crimean-congo hemorrhagic fever virus （CCHFV） is a geographically widespread fatal pathogen Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP （ nucleocapsid protein ） gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum （rabbit） and 2 monoclonal （mAbs） （ 14B7 and 43E5 ） against CCHFV by Western-blot analyses The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein