A simple test for the detection of KPC and metallo-β-lactamase carbapenemase-producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin

• Published in: Clinical microbiology and infection Vol. 17; no. 9; pp. 1438 - 1441
• Main Authors: Pasteran, F., Veliz, O., Faccone, D., Guerriero, L., Rapoport, M., Mendez, T., Corso, A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.09.2011; Blackwell Publishing Ltd; Wiley-Blackwell
• Subjects: 3-Aminophenylboronic acid; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial Proteins - drug effects; Bacterial Proteins - metabolism; beta-Lactamases - drug effects; beta-Lactamases - metabolism; Biological and medical sciences; Boronic Acids - pharmacology; carbapenemase; Cloxacillin; Cloxacillin - pharmacology; dipicolinic acid; Disk Diffusion Antimicrobial Tests - methods; Enzymes; Humans; Infectious diseases; Medical sciences; Meropenem; Metallo- beta -lactamase; Pharmacology. Drug treatments; Picolinic Acids - pharmacology; Pseudomonas aeruginosa; Pseudomonas aeruginosa - classification; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - enzymology; Pseudomonas Infections - microbiology; Sensitivity and Specificity; Thienamycins - pharmacology; Pseudomonas aeruginosa; 3-Aminophenylboronic acid; carbapenemase; dipicolinic acid; cloxacillin; Pseudomonadales; Enzyme; β-Lactams; β-Lactamase; Cloxacillin; Penicillin derivatives; Infection; Carbapenem derivatives; Antibiotic; Bacteria; Hydrolases; Pseudomonadaceae; Meropenem; Antibacterial agent
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2011.03585.x

We evaluated the ability of the combination disk test (CDT) and the Modified Hodge Test (MHT) to discriminate between various carbapenemase-producing Pseudomonas aeruginosa isolates (KPC, n = 36; metallo-β-lactamase (MBL), n = 38) and carbapenemase non-producers (n = 75). For the CDT, the optimal inhibitor concentrations and cut-off values were: 600 µg of 3-aminophenylboronic acid (APB) per disk (an increment of ≥4 mm), 1000 µg of dipicolinic acid (DPA) per disk (an increment of ≥5 mm) and 3000 µg of cloxacillin per disk (an increment of ≥3 mm). APB had excellent sensitivity (97%) and specificity (97%) for the detection of KPC enzymes. DPA detected MBL enzymes with a sensitivity and specificity of 97% and 81%, respectively. The MHT resulted in a low sensitivity (78%) and specificity (57%). The CDT could be very useful in daily practice to provide fast and reliable detection of KPC and MBL carbapenemases among P. aeruginosa isolates.