Graphene-Induced Adsorptive and Optical Artifacts During In Vitro Toxicology Assays

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 9; no. 11; pp. 1921 - 1927
• Main Authors: Creighton, Megan A., Rangel-Mendez, J. Rene, Huang, Jiaxing, Kane, Agnes B., Hurt, Robert H.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 10.06.2013; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Absorption; adsorption; Assaying; Biotechnology; Colorimetry; Dyes; fluorescence; Graphene; In vitro testing; micronutrients; Nanotechnology; Quenching; toxicology

Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the in...