Graphene-Induced Adsorptive and Optical Artifacts During In Vitro Toxicology Assays

Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the in...

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Bibliographic Details
Published inSmall (Weinheim an der Bergstrasse, Germany) Vol. 9; no. 11; pp. 1921 - 1927
Main Authors Creighton, Megan A., Rangel-Mendez, J. Rene, Huang, Jiaxing, Kane, Agnes B., Hurt, Robert H.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 10.06.2013
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
Absorption
adsorption
Assaying
Biotechnology
Colorimetry
Dyes
fluorescence
Graphene
In vitro testing
micronutrients
Nanotechnology
Quenching
toxicology
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Summary:Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the interaction, and/or (3) absorbing or scattering light, which are issues for both colorimetric and fluorescent assays.
Bibliography:istex:4CA793DACD72E66C0FC202FEC8C729B8735C7764
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ArticleID:SMLL201202625
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201202625