Opposite regulation of uncoupling protein 1 and uncoupling protein 3 in vivo in brown adipose tissue of cold-exposed rats

• Published in: FEBS letters Vol. 519; no. 1; pp. 210 - 214
• Main Authors: Jakus, P.B, Sipos, K, Kispal, G, Sandor, A
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 22.05.2002
• Subjects: Acclimatization - physiology; Adipose Tissue, Brown - metabolism; Animals; BAT, brown adipose tissue; Blotting, Northern; Blotting, Western; Brown adipose tissue; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cold Temperature; Frataxin; Glucose transporter 4; Glucose Transporter Type 4; GLUT4, glucose transporter 4; Ion Channels; Iron-Binding Proteins; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mitochondrial Proteins; Monosaccharide Transport Proteins - metabolism; Muscle Proteins; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Rats; Rats, Wistar; RNA, Messenger - metabolism; UCP1, 2, 3, uncoupling protein 1, 2, 3; Uncoupling protein; Uncoupling Protein 1; Uncoupling Protein 3; Brown adipose tissue; GLUT4, glucose transporter 4; Uncoupling protein; Glucose transporter 4; UCP1, 2, 3, uncoupling protein 1, 2, 3; BAT, brown adipose tissue
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(02)02707-2

Earlier we reported a 14-fold increase of glycogen in the brown adipose tissue (BAT) in rats when the animals were placed back from cold to neutral temperature. To elucidate the mechanism, here we compared the level of glucose transporter 4 (GLUT4) protein, uncoupling protein (UCP) 1 and UCP3 mRNA and protein expressions in the BAT under the same conditions. We found that the increased GLUT4 level in cold was maintained during the reacclimation. After 1 week cold exposure the mRNA and protein content of UCP1 increased parallel, while the protein level of UCP3 decreased, contrary to its own mRNA level.