Extraction of methocarbamol from human plasma with a polypyrrole/multiwalled carbon nanotubes composite decorated with magnetic nanoparticles as an adsorbent followed by electrospray ionization ion mobility spectrometry detection

• Published in: Journal of separation science Vol. 37; no. 23; pp. 3518 - 3525
• Main Authors: Saraji, Mohammad, Khayamian, Taghi, Hashemian, Zahra
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.12.2014; Wiley; Wiley Subscription Services, Inc
• Subjects: Adsorbents; Adsorption; Analysis; Biological and medical sciences; blood; Carbon; carbon nanotubes; Chemical synthesis; detection limit; drugs; Extraction; General pharmacology; Humans; infrared spectroscopy; Ion mobility; Ion mobility spectrometry; Ionic mobility; Ionization; Ions; Magnetic nanoparticles; Magnetite Nanoparticles - chemistry; Medical sciences; Methocarbamol; Methocarbamol - blood; Methocarbamol - chemistry; Methocarbamol - isolation & purification; Microscopy; Multiwalled carbon nanotubes; nanocomposites; Nanoparticles; Nanotubes; Nanotubes, Carbon - chemistry; Pharmacology. Drug treatments; Plasma - chemistry; Polymers - chemistry; Polypyrrole; Polypyrroles; Pyrroles - chemistry; scanning electron microscopy; Solid Phase Extraction - instrumentation; Solid Phase Extraction - methods; Sorbents; Spectrometry, Mass, Electrospray Ionization; Spectroscopy; Spectrum analysis; transmission electron microscopy; X-ray diffraction; Solid phase extraction; Biological fluid; Fourier transformation; Chemical analysis; Ion mobility spectrometry; Nanoparticle; Carbon nanotubes; Electrospray; Blood; Chemical enrichment; Blood plasma; Surface structure; Characterization; Multiwalled carbon nanotubes; Sample preparation; Methocarbamol; Antispasmodic agent; Adsorbent; Polypyrrole; Quantitative analysis; Modified material; Human; Validation; Iron II Iron III Oxides; Healthy subject; Muscle relaxant; Magnetic nanoparticles; X ray diffraction; Conducting polymers; Infrared spectrometry; Magnetic particles; Transmission electron microscopy; Multiwalled nanotube; Magnetic separation; Morphology; Preparation; Pyrrole polymer
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201400614

In this work, a polypyrrole/multiwalled carbon nanotubes composite decorated with Fe3O4 nanoparticles was chemically synthesized and applied as a novel adsorbent for the extraction of methocarbamol from human plasma. Electrospray ionization ion mobility spectrometry was used for the determination of the analyte. The properties of the magnetic‐modified adsorbent were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform IR spectroscopy, and X‐ray diffraction. The effects of experimental parameters on the extraction efficiency of the sorbent were investigated. Under the optimized conditions, the linear dynamic range was found to be 2–150 ng/mL with the detection limit of 0.9 ng/mL. The relative standard deviation was 5.3% for three replicate measurements of methocarbamol in plasma sample. The extraction efficiency of the sorbent for the determination of different drugs with various polarities was also compared to that of Fe3O4‐polypyrrole and Fe3O4‐multiwalled carbon nanotubes sorbents. Finally, the method was used for the determination of methocarbamol in blood samples.