Construction of a Collagen-like Protein Based on Elastin-like Polypeptide Fusion and Evaluation of Its Performance in Promoting Wound Healing

In the healing of wounds, human-like collagen (hCol) is essential. However, collagen-based composite dressings have poor stability in vivo, which severely limits their current therapeutic potential. Based on the above, we have developed a recombinant fusion protein named hCol-ELP, which consists of...

Full description

Saved in:
Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 28; no. 19; p. 6773
Main Authors Chen, Yingli, Wu, Yuanyuan, Xiong, Fengmin, Yu, Wei, Wang, Tingting, Xiong, Jingjing, Zhou, Luping, Hu, Fei, Ye, Xianlong, Liang, Xinmiao
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 23.09.2023
MDPI
Subjects
Analysis
Biological activity
Cell growth
Clinical medicine
Collagen
Composite materials
Elastin
elastin-like polypeptide (ELP)
Extracellular matrix
Fibroblasts
fusion protein
Health aspects
human-like collagen (hCol)
Mechanical properties
Molecular weight
Peptides
Physiology
Proteins
Skin
Wound healing
China
United States > US
Online AccessGet full text

Cover

More Information
Summary:In the healing of wounds, human-like collagen (hCol) is essential. However, collagen-based composite dressings have poor stability in vivo, which severely limits their current therapeutic potential. Based on the above, we have developed a recombinant fusion protein named hCol-ELP, which consists of hCol and an elastin-like peptide (ELP). Then, we examined the physicochemical and biological properties of hCol-ELP. The results indicated that the stability of the hCol-ELP fusion protein exhibited a more compact and homogeneous lamellar microstructure along with collagen properties, it was found to be significantly superior to the stability of free hCol. The compound hCol-ELP demonstrated a remarkable capacity to induce the proliferation and migration of mouse embryo fibroblast cells (NIH/3T3), as well as enhance collagen synthesis in human skin fibroblasts (HSF) when tested in vitro. In vivo, hCol-ELP demonstrated significant enhancements in healing rate and a reduction in the time required for scab removal, thereby exhibiting a scar-free healing effect. The findings provide a crucial theoretical foundation for the implementation of an hCol-ELP protein dressing in fields associated with the healing of traumatic injuries.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28196773