β-Carotene promotes the development of NNK-induced small airway-derived lung adenocarcinoma
Abstract Aim β-Carotene has shown cancer-preventive effects in preclinical studies while increasing lung cancer mortality in clinical trials. We have shown that β-carotene stimulates cAMP signalling in vitro . Here, we have tested the hypothesis that β-carotene promotes the development of pulmonary...
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Published in | European journal of cancer (1990) Vol. 45; no. 7; pp. 1257 - 1264 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Kidlington
Elsevier Ltd
01.05.2009
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Abstract Aim β-Carotene has shown cancer-preventive effects in preclinical studies while increasing lung cancer mortality in clinical trials. We have shown that β-carotene stimulates cAMP signalling in vitro . Here, we have tested the hypothesis that β-carotene promotes the development of pulmonary adenocarcinoma (PAC) in vivo via cAMP signalling. Methods PAC was induced in hamsters with the carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), followed by β-carotene for 1.5 years. Incidence, multiplicity and size of lung tumours were recorded, and phosphorylated CREB and ERK1/2 in tumour cells were determined by Western blots. Cyclic AMP in blood cells was analysed by immunoassays, retinoids in serum and lungs by HPLC. Results β-Carotene increased lung tumour multiplicity, lung tumour size, blood cell cAMP, serum and lung levels of retinoids and induced p-CREB and p-ERK1/2 in lung tumours. Conclusions Our data suggest that β-carotene promotes the development of PAC via increased cAMP signalling. |
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ISSN: | 0959-8049 1879-0852 |
DOI: | 10.1016/j.ejca.2008.10.035 |