Renal ischemia-reperfusion leads to long term infiltration of activated and effector-memory T lymphocytes

• Published in: Kidney international Vol. 75; no. 5; pp. 526 - 535
• Main Authors: Ascon, Miguel, Ascon, Dolores B., Liu, Manchang, Cheadle, Chris, Sarkar, Chaitali, Racusen, Lorraine, Hassoun, Heitham T., Rabb, Hamid
• Format: Journal Article
• Language: English
• Published: Basingstoke Elsevier Inc 01.03.2009; Nature Publishing Group; Elsevier Limited
• Subjects: Animals; Biological and medical sciences; cell depletion; Chemotaxis, Leukocyte - immunology; Cytokines - biosynthesis; Flow Cytometry; Immunologic Memory - immunology; Immunophenotyping; Kidney Diseases - immunology; long-term infiltration; Lymphocyte Activation - immunology; Lymphocyte Subsets; Medical sciences; Mice; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; renal ischemia; Renovascular diseases; Reperfusion Injury - immunology; T lymphocytes; T-Lymphocytes - immunology; cell depletion; T lymphocytes; renal ischemia; long-term infiltration; Kidney disease; Activated state; Effectory cell; Nephrology; Urinary system disease; Rodentia; Cardiovascular disease; Long term; Urology; Memory lymphocyte; Vascular disease; Vertebrata; Mammalia; Depletion; Mouse; Animal; T-Lymphocyte; Infiltration; Renal ischemia reperfusion injury
• ISSN: 0085-2538; 1523-1755
• DOI: 10.1038/ki.2008.602

It is well-established that significant ischemia-reperfusion injury during kidney transplantation results in increased incidence of long-term fibrosis and rejection. To test for a role of T cell infiltration and activation following ischemic injury, we induced both bilateral and unilateral renal ischemia in mice, followed by reperfusion, and then isolated mononuclear cells. Analysis of these cells by flow cytometry showed that 2 weeks after bilateral ischemia there was a significant increase of CD8+ T cells. Furthermore, both CD4+ and CD8+ T cells infiltrated the injured kidney 6 weeks after unilateral ischemia. These T cells had increased expression of CD69+ and CD44hiCD62L-, markers of activation and effector-memory, respectively. CD4+NK1.1+ and CD19+ B cells were decreased in percentage both 6 and 11 weeks after bilateral or unilateral injury. There was a significant upregulation of IL-1β, IL-6, TNF-α, IFN-γ, MIP-2, and RANTES expression, measured by real-time PCR, 6 weeks after unilateral renal ischemia, further indicating T cell activation. Depletion of CD4+ and CD8+ T cells before ischemia caused less medullary damage and reduced kidney IFN-γ expression, whereas their depletion following ischemia increased kidney IL-1β; however, depletion of these cells had no effect on histological damage to the kidney. Our study demonstrates that moderate or severe kidney ischemia induces long-term T lymphocyte infiltration and cytokine/chemokine upregulation, leading to kidney structural changes.