Polyoxygenated steroids from the octocoral Leptogorgia punicea and in vitro evaluation of their cytotoxic activity

• Published in: Marine drugs Vol. 12; no. 12; pp. 5864 - 5880
• Main Authors: Moritz, Maria Izabel G, Marostica, Lucas Lourenço, Bianco, Everson M, Almeida, Maria Tereza R, Carraro, João L, Cabrera, Gabriela M, Palermo, Jorge A, Simões, Cláudia M O, Schenkel, Eloir P
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.12.2014; MDPI
• Subjects: A549 cells; Animals; Anthozoa - chemistry; Cell Cycle - drug effects; Cell Line, Tumor; cytotoxic effects; Drug Synergism; Gorgonacea; Humans; Leptogorgia; Leptogorgia punicea; lung cancer cells; Lung Neoplasms - drug therapy; Magnetic Resonance Spectroscopy - methods; Marine; octocoral; Paclitaxel - pharmacology; polyoxygenated steroids; Steroids - chemistry; Steroids - pharmacology
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md12125864

Five new polyoxygenated marine steroids-punicinols A-E (1-5)-were isolated from the gorgonian Leptogorgia punicea and characterized by spectroscopic methods (IR, MS, 1H, 13C and 2-D NMR). The five compounds induced in vitro cytotoxic effects against lung cancer A549 cells, while punicinols A and B were the most active, with IC50 values of 9.7 μM and 9.6 μM, respectively. The synergistic effects of these compounds with paclitaxel, as well as their effects on cell cycle distribution and their performance in the clonogenic assay, were also evaluated. Both compounds demonstrated significant synergistic effects with paclitaxel.