Comparative study of interfacial properties and thermal behaviour of milk fat globules and membrane prepared from ultrasonicated bovine milk

• Published in: Ultrasonics sonochemistry Vol. 102; p. 106755
• Main Authors: Sun, Yanjun, Roos, Yrjö H., Miao, Song
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2024; Elsevier
• Subjects: Animals; DSC; Female; Glycolipids; Glycoproteins; Humans; Infant; Interfacial properties; Lipid Droplets; Milk - chemistry; Milk fat globules; Original; Ultrasonic treatment; X-ray diffraction; X-ray diffraction; Interfacial properties; Milk fat globules; DSC; Ultrasonic treatment
• ISSN: 1350-4177; 1873-2828; 1873-2828
• DOI: 10.1016/j.ultsonch.2024.106755

•Ultrasonic treatment modified the interfacial properties and thermal behaviour of MFGs/MFGM.•40 kHz/ 5 min treatment induced the highest ζ- potential of all samples.•Most significant decrease of interfacial tension (π) was observed in 20 kHz/ 15 min treated samples.•20 kHz and 40 kHz independently or synchronously treatments for 15 min induced the disappearance of α-crystal peaks.•Compared with control, ultrasonic treatment resulted in the appearance of one or two more exothermic events, while undetectable low-melting fractions peaks during the heating stage. Milk fat globules or milk fat globule membranes (MFGs/MFGM) have been added to the infant formula to fortify the phospholipids and narrow the nutritional gap from breast milk. The main aim of this study was to profile the interfacial and thermal properties of MFGs/MFGM prepared from ultrasonicated bovine milk. Bovine milk was sonicated at ultrasonic intensities of 20 kHz and 40 kHz independently or synchronously with the duration time of 0 min (control), 5 min, 10 min, and 15 min (work/rest cycles = 5 s: 3 s). Ultrasonic treatments at 20 kHz/ 5 min and 20 + 40 kHz/ 5 min improved the volume density (%) of smaller particles (1–10 µm) while significantly decreasing the surface hydrophobicity (H0) (p < 0.05). 40 kHz/5 min samples showed significantly higher ζ- potential than the other samples (p < 0.05), which might be because more negative charges were detected. In comparison with control samples, ultrasonic treatments decreased the interfacial tension (π) between the air and MFGs/MFGM liquid phase. 20 kHz ultra-sonicated treatments decreased the diffusion rate (k diff) of MFGs/MFGM interfacial compositions significantly as the duration prolonged from 5 min to 15 min (p < 0.05) but did not affect the adsorption or penetration rate (k a) (p > 0.05). X-ray diffraction (XRD) results showed that α-crystal peaks only existed in control and ultrasonicated 5 min samples but disappeared in all 15 min samples. According to the different scanning calorimetry (DSC), one or two new exothermic events (in the range of 17.29 – 18.81 ℃ and 22.14 – 25.21 ℃) appeared after ultrasonic treatments, which, however, were not found in control samples. Ultrasonic treatments resulted in the low-melting fractions (LMF) (TM1) peaks undetectable in MFGs/MFGM samples in which only peaks of medium-melting fractions (MMF) (TM2) and high-melting fractions (HMF) (TM3) were detected. Compared with the control, both enthalpies of crystallisation (ΔHC) and melting (ΔHM) decreased in ultrasonicated samples. In conclusion, ultrasonic treatment affects the interfacial and thermal properties of MFGs/MFGM.