Yeasts and Lactic Acid Bacteria Mixed-Specie Biofilm Formation is a Promising Cell Immobilization Technology for Ethanol Fermentation

• Published in: Applied biochemistry and biotechnology Vol. 171; no. 1; pp. 72 - 79
• Main Authors: Abe, Atsumu, Furukawa, Soichi, Watanabe, Shinya, Morinaga, Yasushi
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.09.2013; Springer; Springer Nature B.V
• Subjects: Bacillus subtilis; Bacteria; Batch Cell Culture Techniques - methods; Biochemistry; Biofilms; Biofilms - growth & development; Biological and medical sciences; Biotechnology; Cells, Immobilized - metabolism; Cellulose; Chemistry; Chemistry and Materials Science; Contaminants; E coli; Escherichia coli; Ethanol; Ethanol - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; General aspects; Immobilization techniques; Lactic Acid - biosynthesis; Lactobacillus plantarum; Lactobacillus plantarum - cytology; Lactobacillus plantarum - growth & development; Lactobacillus plantarum - metabolism; Lactobacillus plantarum - physiology; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - physiology; Yeast; Yeasts; Ethanol fermentation; Mixed-specie biofilm; Ascomycota; Lactic acid bacteria; Lactobacillus plantarum; Yeast; Ethanol; Immobilization; Fermentation; Fungi; Technology; Biofilm; Bacteria; Lactobacillaceae; Saccharomyces cerevisiae
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-013-0360-6

We previously found that some Saccharomyces cerevisiae and Lactobacillus plantarum remarkably formed mixed-specie biofilm in a static co-culture and deduced that this biofilm had potential as immobilized cells. We investigated the application of mixed-specie biofilm formed by S . cerevisiae BY4741 and L . plantarum HM23 for ethanol fermentation in repeated batch cultures. This mixed-specie biofilm was far abundantly formed and far resistant to washing compared with S . cerevisiae single biofilm. Adopting mixed-specie biofilm formed on cellulose beads as immobilized cells, we could produce enough ethanol from 10 or 20 % glucose during ten times repeated batch cultures for a duration of 10 days. Cell numbers of S . cerevisiae and L . plantarum during this period were stable. In mixed-specie biofilm system, though ethanol production was slightly lower compared to S . cerevisiae single-culture system due to by-production of lactate, pH was stably maintained under pH 4 without artificial control suggesting high resistance to contamination. Inoculated model contaminants, Escherichia coli and Bacillus subtilis , were excluded from the system in a short time. From the above results, it was indicated that the mixed-specie biofilm of S . cerevisiae and L . plantarum was a promising immobilized cell for ethanol fermentation for its ethanol productivity and robustness due to high resistance to contamination.