Expression of methylation-related genes is associated with overall survival in patients with non-small cell lung cancer
The abnormality of DNA methylation is involved in tumour progression, and thus has a modulating effect on clinical outcome of cancer patients. In this study, we measured the mRNA expression levels of three methylation-regulating genes ( DNMT1 , DNMT3b , and MBD2 ) in 148 tumour samples from patients...
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Published in | British journal of cancer Vol. 98; no. 10; pp. 1716 - 1722 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
20.05.2008
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | The abnormality of DNA methylation is involved in tumour progression, and thus has a modulating effect on clinical outcome of cancer patients. In this study, we measured the mRNA expression levels of three methylation-regulating genes (
DNMT1
,
DNMT3b
, and
MBD2
) in 148 tumour samples from patients with non-small cell lung cancer (NSCLC) using quantitative real-time polymerase chain reaction and then determined their prognostic values. Our data showed that the high level of
DNMT1
expression was significantly associated with an increased risk of death in all NSCLC patients (hazard ratio (HR), 1.74; 95% confidence interval (95% CI), 1.04–2.90). However, the high level of
DNMT3b
expression was significantly associated with poor prognosis only in young patients (<65 years). The high level of
MBD2
expression had a significantly reduced risk for death only in male patients and in squamous cell lung carcinoma (SQLC) patients. All three combination groups with
DNMT1
and
DNMT3b
,
DNMT1
and
MBD2
or
DNMT3b
and
MBD2
revealed significant combined effects in male patients and SQLC patients. Our results suggest that DNMT1, DNMT3b, and MBD2 may play important roles in modulating NSCLC patient survival and thus be useful for identifying NSCLC patients who would benefit most from aggressive therapy. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0007-0920 1532-1827 |
DOI: | 10.1038/sj.bjc.6604343 |