Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: Lipidomics analysis of a Diabetes Antibody Standardization Program sample subset

• Published in: Clinical biochemistry Vol. 43; no. 12; pp. 948 - 956
• Main Authors: Sorensen, Christina M., Ding, Jie, Zhang, Qibin, Alquier, Thierry, Zhao, Rui, Mueller, Patricia W., Smith, Richard D., Metz, Thomas O.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.08.2010; Elsevier
• Subjects: Adolescent; Adult; AMT tag approach; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; Capillary liquid chromatography; Child; CHROMATOGRAPHY; Chromatography, Liquid; DESIGN; Diabetes Antibody Standardization Program; DIABETES MELLITUS; Diabetes Mellitus, Type 1 - blood; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Environmental Molecular Sciences Laboratory; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; Humans; ION CYCLOTRON-RESONANCE; Lipidomics; LIPIDS; Lipids - blood; Male; MASS SPECTROMETERS; Mass spectrometry; Medical sciences; PLASMA; REGULATIONS; STANDARDIZATION; Type 1 diabetes; Young Adult; Lipidomics; AMT tag approach; Type 1 diabetes; Capillary liquid chromatography; Mass spectrometry; Diabetes Antibody Standardization Program; Endocrinopathy; Human; Immunopathology; Exploration; Autoimmune disease; Lipids; Early stage; Liquid chromatography; Profile; Blood plasma; Clinical biology; Serum
• ISSN: 0009-9120; 1873-2933; 1873-2933
• DOI: 10.1016/j.clinbiochem.2010.04.075

To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000–2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach. Five hundred fifty-nine lipid features differentiated ( q < 0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing individuals with recently diagnosed type 1 diabetes mellitus. A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization.