Image-Guided Tissue Engineering of Anatomically Shaped Implants via MRI and Micro-CT Using Injection Molding

This study demonstrates for the first time the development of engineered tissues based on anatomic geometries derived from widely used medical imaging modalities such as computed tomography (CT) and magnetic resonance imaging (MRI). Computer-aided design and tissue injection molding techniques have...

Full description

Saved in:
Bibliographic Details
Published inTissue engineering. Part A Vol. 14; no. 7; pp. 1195 - 1202
Main Authors Ballyns, Jeffrey J., Gleghorn, Jason P., Niebrzydowski, Vicki, Rawlinson, Jeremy J., Potter, Hollis G., Maher, Suzanne A., Wright, Timothy M., Bonassar, Lawrence J.
Format Journal Article
LanguageEnglish
Published United States Mary Ann Liebert, Inc 01.07.2008
Subjects
Animals
Cattle
Cell Culture Techniques
Cells, Cultured
CT imaging
Extracellular Matrix - metabolism
Injection molding
Magnetic Resonance Imaging
Menisci, Tibial - cytology
Menisci, Tibial - diagnostic imaging
Menisci, Tibial - metabolism
Methods
NMR
Nuclear magnetic resonance
Physiological aspects
Sheep
Tissue engineering
Tissue Engineering - methods
Tomography
Tomography, X-Ray Computed
Transplants & implants
United States
Online AccessGet full text

Cover

More Information
Summary:This study demonstrates for the first time the development of engineered tissues based on anatomic geometries derived from widely used medical imaging modalities such as computed tomography (CT) and magnetic resonance imaging (MRI). Computer-aided design and tissue injection molding techniques have demonstrated the ability to generate living implants of complex geometry. Due to its complex geometry, the meniscus of the knee was used as an example of this technique's capabilities. MRI and microcomputed tomography (μCT) were used to design custom-printed molds that enabled the generation of anatomically shaped constructs that retained shape throughout 8 weeks of culture. Engineered constructs showed progressive tissue formation indicated by increases in extracellular matrix content and mechanical properties. The paradigm of interfacing tissue injection molding technology can be applied to other medical imaging techniques that render 3D models of anatomy, demonstrating the potential to apply the current technique to engineering of many tissues and organs.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2007.0186