Tomato Juice Supplementation Influences the Gene Expression Related to Steatosis in Rats

• Published in: Nutrients Vol. 10; no. 9; p. 1215
• Main Authors: Elvira-Torales, Laura Inés, Navarro-González, Inmaculada, González-Barrio, Rocío, Martín-Pozuelo, Gala, Doménech, Guillermo, Seva, Juan, García-Alonso, Javier, Periago-Castón, María Jesús
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.09.2018; MDPI
• Subjects: Amino Acids - biosynthesis; Animals; Antioxidants; Apolipoproteins B - metabolism; Biomarkers - metabolism; Biosynthesis; Carotenoids; CD36 antigen; CD36 Antigens - metabolism; Chemical compounds; Diet; Dietary Supplements; Down-Regulation; Fatty acids; Fatty Liver - genetics; Food; Fruit and Vegetable Juices; Gene expression; Gene Expression - physiology; Genes; Glutathione - biosynthesis; Hepatocyte nuclear factor 4; Hepatocyte Nuclear Factor 4 - metabolism; High fat diet; Inflammation; Isoprostanes; Juices; Laboratories; Lipids; Lipoprotein Lipase - metabolism; Lipoproteins; Liver; Liver - metabolism; Liver diseases; Lycopene; Lycopene - metabolism; Lycopersicon esculentum; Male; Malondialdehyde; Malondialdehyde - metabolism; Metabolism; Metabolites; Metabolomics; non-alcoholic fatty liver disease (NAFLD); Nutrition research; Obesity; Oxidation; oxidative biomarkers; Oxidative stress; Oxidative Stress - physiology; quantitative metabolomics; Rats; Rats, Sprague-Dawley; Rodents; Steatosis; Tomatoes; Up-Regulation; United States > US; Indianapolis Indiana; Spain; non-alcoholic fatty liver disease (NAFLD); quantitative metabolomics; oxidative biomarkers; lycopene; gene expression
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu10091215

The objective of this work was to identify the effect of tomato juice on the expression of genes and levels of metabolites related to steatosis in rats. Male Sprague Dawley rats (8 weeks-old) were grouped (6 rats/group) in four experimental groups: NA (normal diet and water), NL (normal diet and tomato juice), HA (high-fat diet and water), and HL (high-fat diet and tomato juice). After an intervention period of 5 weeks, rats were sacrificed and biochemical parameters, biomarkers of oxidative stress, liver metabolites, and gene expression were determined. Although the H diet provoked dislipemia related to steatosis, no changes in isoprostanes or liver malondialdehyde (MDA) were observed. Changes in the gene expression of the HA group were produced by the high consumption of fat, whereas the consumption of tomato juice had different effects, depending on the diet. In the NL group, the genes involved in β-oxidation were upregulated, and in groups NL and HL upregulation of and downregulation of and were observed. In addition, in the HL group the accumulation of lycopene upregulated the genes and , which have been suggested as preventive factors in relation to steatosis. Regarding the metabolomics study, intake of tomato juice stimulated the biosynthesis of glutathione and amino acids of the transulfurization pathway, increasing the levels of metabolites related to the antioxidant response.