Long-term increase of hippocampal excitability by histamine and cyclic AMP

• Published in: Neuropharmacology Vol. 36; no. 11; pp. 1539 - 1548
• Main Authors: Selbach, O., Brown, R.E., Haas, H.L.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.11.1997; Elsevier
• Subjects: Animals; Biogenic Amines - pharmacology; Biological and medical sciences; CA1; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Cyclic AMP - pharmacology; Cyclic AMP-Dependent Protein Kinases - metabolism; Electrophysiology; Excitatory Amino Acid Agonists - pharmacology; Extracellular Space - drug effects; Extracellular Space - enzymology; Fundamental and applied biological sciences. Psychology; Hippocampus - drug effects; Hippocampus - enzymology; Histamine - pharmacology; In Vitro Techniques; low Ca 2+/high Mg 2; LTP; Male; protein kinase A; Rat; Rats; Rats, Sprague-Dawley; Rats, Wistar; Receptors, Histamine H1 - drug effects; Receptors, Histamine H2 - drug effects; Receptors, Histamine H3 - drug effects; Second Messenger Systems - drug effects; Vertebrates: nervous system and sense organs; LTP; CA1; Rat; protein kinase A; low Ca 2+/high Mg 2; Enzyme; Transferases; Rodentia; Central nervous system; Discharge pattern; Cyclic AMP; Electrophysiology; In vitro; Histamine; Signal transduction; Vertebrata; Mammalia; Protein kinase; Animal; Biogenic amine; Histamine receptor; Pyramidal neuron; Hippocampus; Brain (vertebrata)
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/S0028-3908(97)00144-5

The action of histamine (HA) on rat hippocampal CA1 pyramidal cells in vitro was investigated in slices perfused with solution containing 0.2 mM Ca 2+ 4.0 mM Mg 2+ . Extracellular recordings of the spontaneous discharges occurring under these conditions revealed that HA caused a long-lasting increase in cell firing. The HA-effects were dose-dependent, in that low concentrations of HA (0.1–0.5 μM) exhibited an initial transient depression of cell firing and practically no long-lasting action, whereas higher concentrations of HA (1–10 μM) exerted strong, non-declining increases. The H 1-receptor antagonist mepyramine (1 μM) blocked the initial depression of firing and attenuated the long-lasting HA-mediated excitation. Pure H 1-receptor activation, tested with the H 1-receptor agonist 2-(3-fluorphenyl)histamine (1–10 μM) depressed cell firing, similar to the low dose effects of HA. HA-induced excitations were prevented by the H 2-receptor antagonist cimetidine (10–50 μM), and mimicked by the very potent H 2-receptor agonist impromidine (1 or 3 μM) which was, however, less effective compared to equal concentrations of HA. H 3-receptor activation by R-α-methylhistamine had no significant effect on cell firing. Thus, histamine H 1 and H 2 receptors seem to cooperate in producing this long-lasting augmentation of excitability. 8-Bromo-cyclic AMP monophosphate (8-Br-cAMP, 50–100 μM) mimicked the long-term excitation, whereas the adenylyl-cyclase inhibitor 9-tetrahydro-2-furyladenine (THFA, 100–500 μM) or the PKA-inhibitor Rp-adenosine-3′5′-cyclic monophosphate (Rp-cAMPS, 10 μM) blocked it, indicating that the HA-mediated increase of excitability in the hippocampus is dependent on the adenylate cyclase/PKA-signal transduction cascade. dl-2-Amino-5-phosphonopentanoic acid (APV, 50 μM) significantly attenuated the magnitude of the HA-induced enhancement, indicating an NMDA receptor-dependent component. Other biogenic amines, acting through receptors positively coupled to adenylyl cyclase, elicited similar responses as HA, indicating common mechanisms by which these substances modulate excitability in CA1 pyramidal cells.