GH13 amylosucrases and GH70 branching sucrases, atypical enzymes in their respective families

• Published in: Cellular and molecular life sciences : CMLS Vol. 73; no. 14; pp. 2661 - 2679
• Main Authors: Moulis, Claire, André, Isabelle, Remaud-Simeon, Magali
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.07.2016; Springer Nature B.V; Springer Verlag
• Subjects: alternansucrase; Amino Acid Sequence; Amylose; Amylosucrase; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biosynthesis; Branching; Carbohydrates; Cell Biology; Dextran; Dextrans; Enzyme kinetics; Enzymes; Flavonoids; Glucan; Glucosyltransferases - chemistry; Glucosyltransferases - metabolism; Glycosides; Glycosyltransferases - chemistry; Glycosyltransferases - metabolism; Kinetics; Life Sciences; Models, Molecular; Multi-Author Review; Multigene Family; Nucleotides; Polymerization; Polyols; Protein Engineering; Sucrose; Sugar; Branching sucrase glucansucrase; Sucrose-active enzymes; Amylosucrase; Transglucosylation
• ISSN: 1420-682X; 1420-9071; 1420-9071
• DOI: 10.1007/s00018-016-2244-8

Amylosucrases and branching sucrases are α-retaining transglucosylases found in the glycoside-hydrolase families 13 and 70, respectively, of the clan GH-H. These enzymes display unique activities in their respective families. Using sucrose as substrate and without mediation of nucleotide-activated sugars, amylosucrase catalyzes the formation of an α-(1 → 4) linked glucan that resembles amylose. In contrast, the recently discovered branching sucrases are unable to catalyze polymerization of glucosyl units as they are rather specific for dextran branching through α-(1 → 2) or α-(1 → 3) branching linkages depending on the enzyme regiospecificity. In addition, GH13 amylosucrases and GH70 branching sucrases are naturally promiscuous and can glucosylate different types of acceptor molecules including sugars, polyols, or flavonoids. Amylosucrases have been the most investigated glucansucrases, in particular to control product profiles or to successfully develop tailored α-transglucosylases able to glucosylate various molecules of interest, for example, chemically protected carbohydrates that are planned to enter in chemoenzymatic pathways. The structural traits of these atypical enzymes will be described and compared, and an overview of the potential of natural or engineered enzymes for glycodiversification and chemoenzymatic synthesis will be highlighted.