Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete Streptomyces cyaneofuscatus M-169

• Published in: Marine drugs Vol. 16; no. 11; p. 406
• Main Authors: Rodríguez, Víctor, Martín, Jesús, Sarmiento-Vizcaíno, Aida, de la Cruz, Mercedes, García, Luis A, Blanco, Gloria, Reyes, Fernando
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.10.2018; MDPI
• Subjects: anthracimycin; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Aquatic Organisms - chemistry; Bacteria; Biosynthesis; Cantabrian Sea; Chromatography; Congeners; Cytotoxicity; Deep sea; Deep water; deep-sea actinobacteria; Gram-positive bacteria; Gram-Positive Bacteria - drug effects; High performance liquid chromatography; HPLC; Liquid chromatography; Maximum likelihood method; Microbial Sensitivity Tests; Natural products; NMR; Nuclear magnetic resonance; Pathogens; Phylogenetics; Phylogeny; Polyketides - chemistry; Polyketides - isolation & purification; Polyketides - pharmacology; Staphylococcus infections; Streptomyces; Streptomyces - chemistry; Streptomyces - genetics; Streptomyces cyaneofuscatus; Tuberculosis; Streptomyces cyaneofuscatus; Cantabrian Sea; anthracimycin; deep-sea actinobacteria
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md16110406

The potent antimicrobial extract of a culture of the marine derived actinomycete M-169 was fractionated by reversed phase flash chromatography and preparative HPLC to yield the new Gram-positive antibiotic, anthracimycin B ( ), together with its congener, anthracimycin ( ). The structure of the new compound was established by analysis of its ESI-TOF MS and 1D and 2D NMR spectra, and comparison with data published for anthracimycin and anthracimycin BII-2619 ( ). Notably, anthracimycin seemed to be the major and almost unique component of the extract detected by HPLC-UV-MS, making our strain an excellent candidate for further biosynthetic studies of this potent antibiotic.