Credentialing a Preclinical Mouse Model of Alveolar Rhabdomyosarcoma

• Published in: Cancer research (Chicago, Ill.) Vol. 69; no. 7; pp. 2902 - 2911
• Main Authors: NISHIJO, Koichi, CHEN, Qing-Rong, ARONOW, Bruce J, BARR, Frederic G, LOR RANDALL, R, LADANYI, Marc, QUALMAN, Stephen J, RUBIN, Brian P, LEGALLO, Robin D, WANG, Chiayeng, KHAN, Javed, KELLER, Charles, LEI ZHANG, MCCLEISH, Amanda T, RODRIGUEZ, Andrea, MIN JUNG CHO, PRAJAPATI, Suresh I, GELFOND, Jonathan A. L, CHISHOLM, Gary B, MICHALEK, Joel E
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01.04.2009
• Subjects: Alleles; Animals; Antineoplastic agents; Biological and medical sciences; Cyclin-Dependent Kinase Inhibitor p16 - genetics; Disease Progression; Diseases of the osteoarticular system; Forkhead Box Protein O1; Forkhead Transcription Factors - biosynthesis; Forkhead Transcription Factors - genetics; Gene Expression Regulation, Neoplastic; Humans; Medical sciences; Mice; Mice, Knockout; Oncogene Proteins, Fusion - biosynthesis; Oncogene Proteins, Fusion - genetics; Paired Box Transcription Factors - biosynthesis; Paired Box Transcription Factors - genetics; PAX3 Transcription Factor; Penetrance; Pharmacology. Drug treatments; Rhabdomyosarcoma, Alveolar - genetics; Rhabdomyosarcoma, Alveolar - metabolism; Rhabdomyosarcoma, Alveolar - pathology; Transcriptional Activation; Tumor Suppressor Protein p53 - genetics; Tumors; Tumors of striated muscle and skeleton; Animal model; Striated muscle disease; Vertebrata; Mammalia; Sarcoma; Mouse; Rodentia; Preclinical trial; Malignant tumor; Alveolar rhabdomyosarcoma; Cancer
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/0008-5472.can-08-3723

The highly aggressive muscle cancer alveolar rhabdomyosarcoma (ARMS) is one of the most common soft tissue sarcoma of childhood, yet the outcome for the unresectable and metastatic disease is dismal and unchanged for nearly three decades. To better understand the pathogenesis of this disease and to facilitate novel preclinical approaches, we previously developed a conditional mouse model of ARMS by faithfully recapitulating the genetic mutations observed in the human disease, i.e., activation of Pax3:Fkhr fusion gene with either p53 or Cdkn2a inactivation. In this report, we show that this model recapitulates the immunohistochemical profile and the rapid progression of the human disease. We show that Pax3:Fkhr expression increases during late preneoplasia but tumor cells undergoing metastasis are under apparent selection for Pax3:Fkhr expression. At a whole-genome level, a cross-species gene set enrichment analysis and metagene projection study showed that our mouse model is most similar to human ARMS when compared with other pediatric cancers. We have defined an expression profile conserved between mouse and human ARMS, as well as a Pax3:Fkhr signature, including the target gene, SKP2. We further identified 7 "druggable" kinases overexpressed across species. The data affirm the accuracy of this genetically engineered mouse model.