Fitness of Transgenic Anopheles stephensi Mosquitoes Expressing the SM1 Peptide under the Control of a Vitellogenin Promoter
Three transgenic Anopheles stephensi lines were established that strongly inhibit transmission of the mouse malaria parasite Plasmodium berghei. Fitness of the transgenic mosquitoes was assessed based on life table analysis and competition experiments between transgenic and wild-type mosquitoes. Lif...
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Published in | The Journal of heredity Vol. 99; no. 3; pp. 275 - 282 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
The American Genetic Association
01.05.2008
Oxford University Press Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | Three transgenic Anopheles stephensi lines were established that strongly inhibit transmission of the mouse malaria parasite Plasmodium berghei. Fitness of the transgenic mosquitoes was assessed based on life table analysis and competition experiments between transgenic and wild-type mosquitoes. Life table analysis indicated low fitness load for the 2 single-insertion transgenic mosquito lines VD35 and VD26 and no load for the double-insertion transgenic mosquito line VD9. However, in cage experiments, where each of the 3 homozygous transgenic mosquitoes was mixed with nontransgenic mosquitoes, transgene frequency of all 3 lines decreased with time. Further experiments suggested that reduction of transgene frequency is a consequence of reduced mating success, reduced reproductive capacity, and/or insertional mutagenesis, rather than expression of the transgene itself. Thus, for transgenic mosquitoes released in the field to be effective in reducing malaria transmission, a driving mechanism will be required. |
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Bibliography: | Chaoyang Li and Mauro T. Marrelli contributed equally to this work. ark:/67375/HXZ-J3Z5F709-4 istex:32684C89372029E5313479021E6F51A4CCAFEF39 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-1503 1465-7333 1471-8505 |
DOI: | 10.1093/jhered/esn004 |