Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells

• Published in: Journal of biomedical materials research. Part A Vol. 103; no. 6; pp. 2172 - 2175
• Main Authors: Ribeiro, Clarisse, Pärssinen, Jenita, Sencadas, Vítor, Correia, Vítor, Miettinen, S., Hytönen, Vesa P., Lanceros-Méndez, Senentxu
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.06.2015; Wiley Subscription Services, Inc
• Subjects: Adipose Tissue - cytology; Biocompatibility; Biomedical materials; Bones; Cell Differentiation - drug effects; Cells, Cultured; Differentiation; dynamic conditions; Dynamics; Electric Stimulation; electroactive polymer; Human; Humans; osteogenesis; Osteogenesis - drug effects; Polyvinyls - pharmacology; stem cell; Stem cells; Stem Cells - cytology; Tissue engineering; electroactive polymer; dynamic conditions; osteogenesis; stem cell
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35368

This work reports on the influence of the substrate polarization of electroactive β‐poly(vinylidene fluoride) (β‐PVDF) on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β‐PVDF surfaces (non‐poled and “poled −”) adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. “Poled −” β‐PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2172–2175, 2015.