Coaggregation ability of Weissella cibaria isolates with Fusobacterium nucleatum and their adhesiveness to epithelial cells

• Published in: FEMS microbiology letters Vol. 253; no. 2; pp. 323 - 329
• Main Authors: Kang, Mi-Sun, Na, Hee-Sam, Oh, Jong-Suk
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 15.12.2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Adhesion tests; Adhesiveness; Amino Acids; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - physiology; Bacterial Adhesion - drug effects; Bacterial Typing Techniques; Bacteriology; Biofilms - growth & development; Biological and medical sciences; Child; Child, Preschool; Coaggregation; Electrophoresis, Polyacrylamide Gel; Epithelial cell; Epithelial cells; Epithelial Cells - microbiology; Flora; Fundamental and applied biological sciences. Psychology; Fusobacterium nucleatum; Fusobacterium nucleatum - physiology; Growth, nutrition, cell differenciation; Hot Temperature; Humans; Lithium chloride; Membrane Proteins; Microbiology; Morphology, structure, chemical composition; Mouth - microbiology; Oral cavity; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phylogeny; Probiotics; Pronase; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Weissella cibaria; Weissella cibaria; Epithelial cell; Fusobacterium nucleatum; Coaggregation; Adhesiveness; Aggregation; Photography; Adhesiveness: Weissella cibaria; Phylogenetic tree; Bacteria; Electrophoresis; Adhesion; Bacteroidaceae; Weissella
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2005.10.002

In the present study, we evaluated the ability of Weissella cibaria isolated from the oral cavity to coaggregate with Fusobacterium nucleatum, and the adhesiveness of these strains to epithelial cells. W. cibaria efficiently coaggregated with F. nucleatum, and adhered to epithelial cells. We tested the effects of various factors on the coaggregation. The coaggregation and adhesiveness of W. cibaria disappeared upon exposure to pronase or LiCl, suggesting that proteinaceous components on the surface of W. cibaria mediated the coaggregation and adhesiveness. In conclusion, W. cibaria may serve as a potential probiotic with the ability to establish an oral flora protecting against oral pathogens.