Ly‐6A is required for T cell receptor expression and protein tyrosine kinase fyn activity

• Published in: The EMBO journal Vol. 13; no. 9; pp. 2167 - 2176
• Main Authors: Lee, S.K., Su, B., Maher, S.E., Bothwell, A.L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 01.05.1994
• Subjects: Animals; Antigens, Ly - genetics; Antigens, Ly - metabolism; Base Sequence; Biological and medical sciences; Clone Cells; Cloning, Molecular; DNA Primers; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Introns; Lymphocyte Activation; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Mice; Molecular Sequence Data; Protein-Tyrosine Kinases - metabolism; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-fyn; Receptors, Antigen, T-Cell - biosynthesis; RNA, Antisense - genetics; Signal Transduction; T-Lymphocytes - metabolism; Transfection; T cell receptor; Enzyme; Immunomodulation; Transferases; Rodentia; Gene expression; Cell surface; Signal transduction; Vertebrata; Mammalia; Enzymatic activity; Mouse; T-Lymphocyte; Protein-tyrosine kinase
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1002/j.1460-2075.1994.tb06493.x

To characterize the function of the Ly‐6A antigen in T cell activation, antisense Ly‐6 RNA was expressed in a stably transfected antigen‐specific T cell clone. Reduced Ly‐6A expression results in inhibition of responses to antigen, anti‐TCR (anti‐T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly‐6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR beta chain. These data demonstrate that Ly‐6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.